Anti-PD-1 (RMP1-14)

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$250.00 - $4,800.00

Explore insights with the Anti-PD-1 (RMP1-14) Monoclonal Antibody—a reliable tool for nuanced research in immune modulation studies.

SKU:
ICH1132
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Details

Anti-PD-1 (RMP1-14)

Product Specific Citations:

Benguigui et al. Interferon-stimulated neutrophils as a predictor of immunotherapy response. Cancer Cell. 4:S1535-6108(23)00433-6.

Wu et al. Rational design of synthetically tractable HDAC6/HSP90 dual inhibitors to destroy immune-suppressive tumor microenvironment. Journal of Advanced Research (2023)

Kauffman et al. PSGL-1 Blockade Induces Classical Activation of Human Tumor-Associated Macrophages. Cancer Research Communications (2023)

Liang et al. SIX4 Controls STING Expression Enhancing anti-PD-1 Efficacy. bioRxiv (2023)

Chen et al. A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles. Nature Communications (2023)

Chuah et al. Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma. Journal of Hepatology (2022)

Liu et al. PD-1 Blockade Restores the Proliferation of Peripheral Blood Lymphocyte and Inhibits Lymphocyte Apoptosis in a BALB/c Mouse Model of CP BVDV Acute Infection. Frontiers in Immunology (2021)

Bio X Cell:

ichorbio's anti-PD-1 (RMP1-14) antibody is up to 50% cheaper for academia & non-profits and up to 68% cheaper for industry than the equivalent low endotoxin version from Bio X Cell (BE0146). ICH1132UL is up to 7% cheaper for academia and up to 40% cheaper for industry compared to Bio X Cell's ultra-low endotoxin version (BP0146).
 
ichorbio Benefits:

ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is manufactured in a cGMP compliant facility. ichorbio has the folowing options:

ichorbio: the best antibodies for in vivo research.

Related Products

Isotype Control:

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Mouse anti Mouse version:

Mouse Anti-Mouse PD-1 Antibody (RMP1-14) [ICH1182]

Mouse anti-mouse Fc Silenced version: Antibodies against the same target:

Anti-PD-1 In Vivo Antibody - Low Endotoxin [29F.1A12] (ICH1091)

Product Information

Size:

ichorbio's RMP1-14 in vivo antibody is available in the following sizes: 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures bulk multi-gram amounts of our anti-PD-1 RMP1-14 clone - please contact us for pricing.

Target:

PD-1

Clone:

RMP1-14

Isotype:

Rat IgG2a

Other Names:

Programmed cell death protein 1, Pdcd1, CD279

Uniprot:

Q02242

Host:

Rat

Species Reactivity:

Mouse, Rat

Specificity:

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

Subset of double negative thymocytes, activated T and B cells

Background:

CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.

Immunogen:

Mouse PD-1 transfected BHK cells

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC >98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

<0.05 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low & Extremely Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:

  • Mycoplasma spp
  • Mycoplasma pulmonis
  • Pneumonia virus of mice
  • Kilham's rat virus
  • Toolan's H1 virus
  • Rat parvovirus
  • Lymphocytic choriomeningitis virus
  • Rat cytomegalovirus
  • Sendai virus
  • Rat coronavirus
  • Sialodacryoadenitis virus
  • Rat minute virus
  • Seoul virus
  • Mouse adenovirus
  • Reovirus 3
  • Rat theilovirus
Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Blocking, Flow Cytometry, Functional Assays

How much RMP1-14 to use in vivo:

Blocking: We recommend using between 2.50 or 7.50 mg/kg when performing in vivo research using ichorbio’s PD-1 low endotoxin antibody clone RMP1-14. This range is based off the most recent publication data using the RMP1-14 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Alternative Names:
  • CD279 antibody
  • CD279 antigen antibody
  • hPD 1 antibody
  • hPD l antibody
  • hPD-1 antibody
  • hSLE1 antibody
  • PD 1 antibody
  • PD-1 antibody
  • PD1 antibody
  • PDCD 1 antibody
  • PDCD1 antibody
  • Programmed cell death 1 antibody
  • Programmed cell death 1 protein antibody
  • Programmed cell death protein 1 antibody
  • Protein PD 1 antibody
  • Protein PD-1 antibody
  • SLEB2 antibody
  • Systemic lupus erythematosus susceptibility 2 antibody

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