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Anti-PD-1 (RMP1-14)

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SKU

ICH1132

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$150.00 - $18,910.00

Anti-PD-1 (RMP1-14)

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Product Specific Citations

Chuah S, Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma. Journal of Hepatology, 2022, 77, 3: 683-694.

Wu TY, 2023, Rational design of synthetically tractable HDAC6/HSP90 dual inhibitors to destroy immune-suppressive tumor microenvironment. Journal of Advanced Research, 2023, 46: 159-171.

Liu Y. et al. PD-1 Blockade Restores the Proliferation of Peripheral Blood Lymphocyte and Inhibits Lymphocyte Apoptosis in a BALB/c Mouse Model of CP BVDV Acute Infection. Front. Immunol, 2021, 12:727254.

Kauffman et al. PSGL-1 Blockade Induces Classical Activation of Human Tumor-Associated Macrophages. Cancer Research Communications, 2023, 3 (10): 2182–2194.

Liang et al. SIX4 Controls STING Expression Enhancing anti-PD-1 Efficacy. Cancer Res Commun. 2023, 3(11):2412-2419.

Xu et al. One-carbon unit supplementation fuels tumor-infiltrating T cells and augments checkpoint blockade. Cell Chem Biol, 2023, 31, 5: 932-943.e8

Benguigui et al. Interferon-stimulated neutrophils as a predictor of immunotherapy response. Cancer Cell, 2024, 42, 253–265

Shyr, C.-R. et al. Evaluating Therapeutic Efficacy of Intravesical Xenogeneic Urothelial Cell Treatment Alone and in Combination with Chemotherapy or Immune Checkpoint Inhibition in a Mouse Non-Muscle-Invasive Bladder Cancer Model. Cancers, 2025; 17(15):24482025, 17, 2448.

Roichman et al. Dietary Fiber Lacks a Consistent Effect on Immune Checkpoint Blockade Efficacy Across Diverse Murine Tumor Models. Cancer Res, 2025, 85 (17): 3335–3347.

Braun, LM. et al. Adiponectin reduces immune checkpoint inhibitor-induced inflammation without blocking anti-tumor immunity. Cancer Cell, 2024, 43, 2: 269 - 291.e19

Braun LM, Zeiser, R. Protocol to study in vivo organ-specific migration of apoptotic splenocytes in mice with tumor and immune checkpoint inhibitor-induced colitis, STAR Protocols, 2025, 6, 2: 103878.

Levin, Sapir et al. Immature monocytic cells within tumors differentiate into immunosuppressive cells in resistant tumors to immunotherapy. iScience, 2025, 28, 8: 113141

Patel, A. et al. Targeting Neuronal Nitric Oxide Synthase (nNOS) as a Novel Approach to Enhancing the Anti-Melanoma Activity of Immune Checkpoint Inhibitors. Pharmaceutics, 2025, 17, 691.

Species Reactivity

Mouse

Target

PD-1

Concentration

>1mg/ml

Isotype

Rat IgG2a Kappa

Host

Rat

Related Antibody

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)

Antibodies Against The Same Target

Aggregation

Aggregation level ≤ 5%

Immunogen

Mouse PD-1 transfected BHK cells

Antigen Distribution

Subset of double negative thymocytes, activated T and B cells

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Uniprot

Q02242

Gene Id

18566

Rrid

RRID:AB_2921498

Shipping Conditions

Blue ice

Background

RMP1-14 is a rat IgG2a, kappa monoclonal antibody raised against mouse PD-1 (Pdcd1, CD279; UniProt Q02242). It is one of the most widely adopted clones for functional interrogation of the PD-1 pathway in mouse models, recognizing the extracellular domain of mouse PD-1 and blocking its engagement by the ligands PD-L1 (CD274) and PD-L2 (CD273). The rat IgG2a backbone is a common choice for in-vivo mouse work, and RMP1-14 is routinely used for checkpoint-blockade studies, alone or combined with other immunomodulatory or targeted agents. This preparation is supplied at low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and in bulk milligram-to-gram quantities, making it suited to in-vivo dosing, ex-vivo functional assays, and larger experimental cohorts. Validated applications include blocking, flow cytometry, functional assays, and Western blot. For research use only (RUO); not for diagnostic or therapeutic use. As with any lot and model, users should validate performance under their own experimental conditions. PD-1 (programmed cell death protein 1; CD279) is an inhibitory immune-checkpoint receptor of the CD28/CTLA-4 immunoglobulin superfamily, expressed on activated T cells and, to varying degrees, B cells, NK cells, and myeloid subsets. Its expression is induced upon antigen-driven lymphocyte activation. Engagement by its ligands PD-L1 (CD274) and PD-L2 (CD273) recruits the phosphatase SHP-2 (PTPN11) to the receptor's cytoplasmic ITIM/ITSM motifs, dampening proximal TCR and CD28 signaling and thereby limiting T-cell activation, proliferation, and cytokine production. This axis is essential for peripheral tolerance and restraining immunopathology, but tumors and chronic infections co-opt it to drive T-cell exhaustion and immune evasion. Blockade of PD-1 or its ligands can restore effector T-cell function, the rationale underpinning checkpoint-blockade immunotherapy.

Other Names

Programmed cell death protein 1, Pdcd1, CD279, RMP1 14, RMP114

Clone

RMP1-14

Specificity

Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

Purification Method

This monoclonal antibody was purified using Protein G

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Storage

anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.

Applications

Western Blot, Blocking, Flow Cytometry, Functional Assays

Application Notes

Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Bio X Cell Code

BE0146, BP0146

Suggested H1

Anti-PD-1/CD279 (RMP1-14)

Faq (q&a)

Q: Is clone RMP1-14 suitable for in vivo use?

A: Yes — RMP1-14 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo PD-1 studies as well as in vitro work.

Q: Can I order RMP1-14 in bulk or gram quantities?

A: Yes, this anti-mouse PD-1 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone RMP1-14 target?

A: It is an anti-mouse PD-1 monoclonal antibody that binds PD-1 and is used in functional and blocking studies; for research use only (RUO).

More Information

ichorbio Benefits:

ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is manufactured in a cGMP compliant facility. ichorbio has the folowing options:

Low Endotoxin: <1EU/mg
Ultra low endotoxin:<0.5EU/mg
Extremely low endotoxin: 0.05EU/mg
5mg/ml concentration
Mouse anti-mouse PD-1 (RMP1-14)
Mouse anti-mouse PD-1 (RMP1-14) Fc Silenced.
Click here for ichorbio's RMP1-14 antibodies and here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars.

ichorbio: the best antibodies for in vivo research.

Related Products

Isotype Control:

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Mouse anti Mouse version:

Mouse Anti-Mouse PD-1 Antibody (RMP1-14) [ICH1182]

Mouse anti-mouse Fc Silenced version:

Mouse Anti-Mouse PD-1 Antibody (RMP1-14) D265A [ICH1182D265A]

Antibodies against the same target:

Anti-PD-1 In Vivo Antibody - Low Endotoxin [29F.1A12] (ICH1091)

Product Information

Size:

ichorbio's RMP1-14 in vivo antibody is available in the following sizes: 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures bulk multi-gram amounts of our anti-PD-1 RMP1-14 clone - please contact us for pricing.

Uniprot:

Q02242

Antigen Distribution:

Subset of double negative thymocytes, activated T and B cells

Immunogen:

Mouse PD-1 transfected BHK cells

Purity:

>95% by SDS-PAGE and HPLC >98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

<0.05 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low & Extremely Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:

Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Blocking, Flow Cytometry, Functional Assays

How much RMP1-14 to use in vivo:

Blocking: We recommend using between 2.50 or 7.50 mg/kg when performing in vivo research using ichorbio’s PD-1 low endotoxin antibody clone RMP1-14. This range is based off the most recent publication data using the RMP1-14 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Alternative Names: