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Anti-Mouse NK1.1 (PK136) In Vivo Antibody - Low Endotoxin
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Bulk anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136)
Bio X Cell:
ICH1128 is up to 37% cheaper for industry than the equivalent low endotoxin product from Bio X Cell (BE0036). ICH1128UL (ultra-low endotoxin) is up to 36% cheaper for industry than the same format from Bio X Cell (BP0036).
Product Benefits:
ichorbio's anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) is manufactured in a cGMP compliant facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Target:NK1.1
Clone:PK136
Size:ichorbio's PK136 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-NK1.1 PK136 clone - please contact us for pricing.
Isotype:Mouse IgG2a
Other Names:Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Host:Mouse
Species Reactivity:Mouse
Specificity:Anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1
Purification Method:This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136.
Background:NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
Immunogen:NK-1+ cells from mouse spleen and bone marrow
Concentration:1.0 - 5.0 mg/ml
Formulation:0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)
Storage:This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen)
Application Notes:Each investigator should determine their own optimal working dilution for specific applications.
Use:Products are for research use only.
Isotype Control:Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)
Alternative Names:- CD161 antigen like family member C antibody
- CD161 antigen-like family member C antibody
- CD161c antibody
- Killer cell lectin-like receptor subfamily B member 1C antibody
- Klrb1c antibody
- Klrb1f antibody
- KLRBC_MOUSE antibody
- Ly-55c antibody
- Ly55c antibody
- Lymphocyte antigen 55c antibody
- Lymphocyte antigen 59 antibody
- Natural killer cell surface protein P1-40 antibody
- NK1.1 antibody
- NKR P1.9 antibody
- NKR P1C antibody
- NKR-P1 40 antibody
- NKR-P1.9 antibody
- NKR-P1C antibody
- NKRP1 antibody
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Mouse IgG2a |
| Host | Mouse |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen) |
| Storage | anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at ≤ 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Anti-NK1.1 In Vivo Antibody - Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1 |
| Species Reactivity | Mouse |
| Target | NK1.1 |
| Shipping Conditions | Blue ice |
| background | NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-_ production, and cytotoxic |
| Other names | Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c |
| clone | PK136 |
| UniProt | P27814 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative us |
| Immunogen | NK-1+ cells from mouse spleen and bone marrow |
| Aggregation | Aggregation level ≤ 5% |
| Related Antibody | Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin [PK136] (ICH1128UL) |
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