Anti-Mouse Ly-6G/Ly-6C (RB6-8C5) In Vivo Antibody - Low Endotoxin

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ICH1131
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Anti-Mouse Ly-6G/Ly-6C (RB6-8C5) In Vivo Antibody - Low Endotoxin

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Concentration 0.5 mg/ml
Isotype Rat IgG2b
Host Rat
Use Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes Each investigator should determine their own optimal working dilution for specific applications.
Applications Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunocytochemistry, Immunofluorescence, In Situ Hybridization
Storage anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin (RB6-8C5) is stable for at least four (4) week when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at ≤ 20°C in a manual defrost freezer. Avoid Repeated
Endotoxin ≤ 1.0 EU/mg as determined by the LAL method
Purity >95% by SDS-PAGE and HPLC
Formulation No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Purification Method This monoclonal antibody was purified using Protein G
Specificity Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes Mouse Granulocytes
Species Reactivity Mouse
Target Ly-6G/Ly-6C
Shipping Conditions Blue ice
background Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of th
Other names Ly-6G/LY-6C, Gr-1, Myeloid
clone RB6-8C5
Buffer ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.
Immunogen Raised against granulocytes of mouse origin
Aggregation Aggregation level ≤ 5%
Antibodies against the same target Anti-Ly-6C In Vivo Antibody - Low Endotoxin [HK1.4] (ICH1111), Anti-Ly-6C In Vivo Antibody - Low Endotoxin [7B10] (ICH1095), Anti-Ly-6G In Vivo Antibody - Low Endotoxin [1A8] (ICH1089), Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin [1A8] (ICH1089)
Related Antibody Anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin [RB6-8C5] (ICH1131UL)
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