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Anti-PD-1 (RMP1-14)

Name: Anti-PD-1 (RMP1-14)
SKU: ICH1132
Price: 150 USD
Availability: InStock

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SKU

ICH1132

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$150.00 - $18,910.00

Anti-PD-1 (RMP1-14)

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Product Specific Citations

Chuah S, Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma. Journal of Hepatology, 2022, 77, 3: 683-694.

Wu TY, 2023, Rational design of synthetically tractable HDAC6/HSP90 dual inhibitors to destroy immune-suppressive tumor microenvironment. Journal of Advanced Research, 2023, 46: 159-171.

Liu Y. et al. PD-1 Blockade Restores the Proliferation of Peripheral Blood Lymphocyte and Inhibits Lymphocyte Apoptosis in a BALB/c Mouse Model of CP BVDV Acute Infection. Front. Immunol, 2021, 12:727254.

Kauffman et al. PSGL-1 Blockade Induces Classical Activation of Human Tumor-Associated Macrophages. Cancer Research Communications, 2023, 3 (10): 2182–2194.

Liang et al. SIX4 Controls STING Expression Enhancing anti-PD-1 Efficacy. Cancer Res Commun. 2023, 3(11):2412-2419.

Xu et al. One-carbon unit supplementation fuels tumor-infiltrating T cells and augments checkpoint blockade. Cell Chem Biol, 2023, 31, 5: 932-943.e8

Benguigui et al. Interferon-stimulated neutrophils as a predictor of immunotherapy response. Cancer Cell, 2024, 42, 253–265

Shyr, C.-R. et al. Evaluating Therapeutic Efficacy of Intravesical Xenogeneic Urothelial Cell Treatment Alone and in Combination with Chemotherapy or Immune Checkpoint Inhibition in a Mouse Non-Muscle-Invasive Bladder Cancer Model. Cancers, 2025; 17(15):24482025, 17, 2448.

Roichman et al. Dietary Fiber Lacks a Consistent Effect on Immune Checkpoint Blockade Efficacy Across Diverse Murine Tumor Models. Cancer Res, 2025, 85 (17): 3335–3347.

Braun, LM. et al. Adiponectin reduces immune checkpoint inhibitor-induced inflammation without blocking anti-tumor immunity. Cancer Cell, 2024, 43, 2: 269 - 291.e19

Braun LM, Zeiser, R. Protocol to study in vivo organ-specific migration of apoptotic splenocytes in mice with tumor and immune checkpoint inhibitor-induced colitis, STAR Protocols, 2025, 6, 2: 103878.

Levin, Sapir et al. Immature monocytic cells within tumors differentiate into immunosuppressive cells in resistant tumors to immunotherapy. iScience, 2025, 28, 8: 113141

Patel, A. et al. Targeting Neuronal Nitric Oxide Synthase (nNOS) as a Novel Approach to Enhancing the Anti-Melanoma Activity of Immune Checkpoint Inhibitors. Pharmaceutics, 2025, 17, 691.

Species Reactivity

Mouse

Target

PD-1

Concentration

1.0 - 5.0 mg/ml

Isotype

Rat IgG2a Kappa

Host

Rat

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Application Notes

Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications.

Applications

Western Blot, Blocking, Flow Cytometry, Functional Assays

Storage

anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purification Method

This monoclonal antibody was purified using Protein G

Specificity

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

Shipping Conditions

Blue ice

Background

PD-1 (CD279) is an inhibitory checkpoint receptor on T cells whose engagement by PD-L1 or PD-L2 limits activation and contributes to T-cell exhaustion. Clone RMP1-14 is one of the most widely used rat IgG2a kappa monoclonal antibodies to mouse PD-1, validated for Western blot, blocking, flow cytometry and functional assays. Anti-PD-1 RMP1-14 is supplied in a low-endotoxin format suitable for in vivo use and produced in scalable milligram (mg) to gram (g) quantities for bulk and high-throughput laboratories. As a non-therapeutic, research-use-only (RUO) reagent, RMP1-14 provides reproducible performance for checkpoint-blockade research in the mouse.

Other Names

Programmed cell death protein 1, Pdcd1, CD279

Clone

RMP1-14

Uniprot

Q02242

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Antigen Distribution

Subset of double negative thymocytes, activated T and B cells

Immunogen

Mouse PD-1 transfected BHK cells

Aggregation

Aggregation level ≤ 5%

Related Antibody

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)

More Information

ichorbio Benefits:

ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is manufactured in a cGMP compliant facility. ichorbio has the folowing options:

Low Endotoxin: <1EU/mg
Ultra low endotoxin:<0.5EU/mg
Extremely low endotoxin: 0.05EU/mg
5mg/ml concentration
Mouse anti-mouse PD-1 (RMP1-14)
Mouse anti-mouse PD-1 (RMP1-14) Fc Silenced.
Click here for ichorbio's RMP1-14 antibodies and here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars.

ichorbio: the best antibodies for in vivo research.

Related Products

Isotype Control:

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Mouse anti Mouse version:

Mouse Anti-Mouse PD-1 Antibody (RMP1-14) [ICH1182]

Mouse anti-mouse Fc Silenced version:

Mouse Anti-Mouse PD-1 Antibody (RMP1-14) D265A [ICH1182D265A]

Antibodies against the same target:

Anti-PD-1 In Vivo Antibody - Low Endotoxin [29F.1A12] (ICH1091)

Product Information

Size:

ichorbio's RMP1-14 in vivo antibody is available in the following sizes: 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures bulk multi-gram amounts of our anti-PD-1 RMP1-14 clone - please contact us for pricing.

Uniprot:

Q02242

Antigen Distribution:

Subset of double negative thymocytes, activated T and B cells

Immunogen:

Mouse PD-1 transfected BHK cells

Purity:

>95% by SDS-PAGE and HPLC >98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

<0.05 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low & Extremely Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:

Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Blocking, Flow Cytometry, Functional Assays

How much RMP1-14 to use in vivo:

Blocking: We recommend using between 2.50 or 7.50 mg/kg when performing in vivo research using ichorbio’s PD-1 low endotoxin antibody clone RMP1-14. This range is based off the most recent publication data using the RMP1-14 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Alternative Names: