Bulk Anti-Human CD3 (UCHT-1) Antibody

Gambles, MT et al. Two-component T-cell immunotherapy enables antigen pre-targeting to reduce cytokine release without forfeiting efficacy, Nanomedicine: Nanotechnology, Biology and Medicine, Volume 67, 2025, 102825.

Li, S. et al. Self-Assembling Multi-Antigen T Cell Hybridizers for Precision Immunotherapy of Multiple Myeloma. Adv. Healthcare Mater, 2025, e02156.

Human

CD3

>1mg/ml

Mouse IgG1

Mouse

Bulk Anti-Human CD3 (OKT-3) Antibody (ICH1257), Muromonab Biosimilar - Research Grade (ICH5024), Mosunetuzumab Biosimilar - Research Grade (ICH5026), Teplizumab Biosimilar - Research Grade (ICH5101), Foralumab Biosimilar - Research Grade (ICH5156), Otelixizumab Biosimilar - Research Grade (ICH5171), Dafsolimab Biosimilar - Research Grade (ICH5460), Emirodatamab Biosimilar - Research Grade (ICH5520), Visilizumab Biosimilar - Research Grade (ICH5982)

Aggregation level ≤ 5%

Unknown

The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P07766

916

Blue ice

This is bulk anti-human CD3 clone UCHT-1, a mouse IgG1 monoclonal raised against human CD3 (UniProt P07766, the CD3-epsilon subunit). UCHT-1 is one of the most widely used and best-characterised anti-human CD3 clones, recognising the epsilon chain of the TCR/CD3 complex on mature human T cells. It is validated across a broad application range, including flow cytometry, immunoprecipitation, western blot, frozen-section IHC, CyTOF/mass cytometry, and functional T-cell assays. Supplied in low-endotoxin, bulk (mg to gram) format for research use only, it is well suited to functional and high-throughput work where endotoxin, aggregation, and lot consistency matter, such as plate-bound or soluble T-cell activation, ex vivo expansion protocols, and immune-monitoring panels. Because the target is human and this is a human-reactive research reagent, it is intended for in-vitro, ex-vivo, and human-sample applications rather than mouse in-vivo dosing models. The bulk, low-endotoxin presentation makes it economical for large screens and repeated functional assays. CD3 is the invariant signalling module of the T-cell receptor (TCR) complex, composed of the CD3-gamma, CD3-delta, and CD3-epsilon chains together with a zeta-chain homodimer, assembled non-covalently around the antigen-binding TCR-alpha/beta (or gamma/delta) heterodimer. Clone UCHT-1 binds the CD3-epsilon subunit encoded near UniProt P07766. While the TCR provides antigen specificity, it lacks intrinsic signalling capacity; the CD3 chains carry the immunoreceptor tyrosine-based activation motifs (ITAMs) that, upon TCR engagement, are phosphorylated by Lck and recruit ZAP-70 to initiate downstream activation, calcium flux, and proliferation. CD3 is expressed exclusively on T lineage cells, making it the definitive pan-T-cell marker in immunophenotyping. Antibodies to CD3-epsilon such as UCHT-1 can crosslink the complex to trigger TCR-independent T-cell activation, which underlies their use in functional stimulation assays.

CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, UCHT 1, UCHT1

UCHT-1

Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR)

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Immunoprecipitation, Western Blot, IHC (Frozen), CyTOF, Functional Assays

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin. Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0231

Anti-Human CD3/CD3E (UCHT-1) Antibody

Q: Is clone UCHT-1 suitable for in vivo use?

A: Yes, UCHT-1 is supplied in a low-endotoxin, in vivo-grade format suitable for functional CD3 studies as well as in vitro work.

Q: Can I get clone UCHT-1 anti-CD3 in gram quantities?

A: Yes, it is produced in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What is clone UCHT-1 used for?

A: UCHT-1 is an anti-human CD3 monoclonal antibody used to study and engage the T-cell receptor complex; for research use only.