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Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Low Endotoxin

Name: Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Low Endotoxin
SKU: ICH1091
Price: 150 USD
Availability: InStock

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SKU

ICH1091

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$150.00 - $23,460.00

Bulk anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12)

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Product Specific Citations

Majewska, J., Agrawal, A., Mayo, A. et al. p16-dependent increase of PD-L1 stability regulates immunosurveillance of senescent cells. Nat Cell Biol, 2024, 26, 1336–1345, .

Liang B. et al. SIX4 Controls Anti-PD-1 Efficacy by Regulating STING Expression. Cancer Research Communications, 2023, 3 (11): 2412–2419.

Species Reactivity

Mouse

Target

PD-1

Concentration

1.0 - 5.0 mg/ml

Isotype

Rat IgG2a Kappa

Host

Rat

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Application Notes

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.

Applications

Flow Cytometry, Western Blot, Blocking, IHC (Frozen)

Storage

anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purification Method

This monoclonal antibody was purified using Protein G

Specificity

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Shipping Conditions

Blue ice

Background

PD-1 (CD279) is an inhibitory checkpoint receptor on activated and exhausted T cells that limits responses upon engagement of PD-L1 or PD-L2. Clone 29F.1A12 is a rat IgG2a kappa monoclonal antibody to mouse PD-1, validated for flow cytometry, Western blot, blocking and frozen IHC. Anti-mouse PD-1 29F.1A12 is supplied in a low-endotoxin format suitable for in vivo use and manufactured in scalable milligram (mg) to gram (g) quantities for bulk and high-throughput laboratories. As a non-therapeutic, research-use-only (RUO) reagent, 29F.1A12 provides reproducible performance for checkpoint-blockade and T-cell-exhaustion research in the mouse.

Other Names

Programmed cell death protein 1, Pdcd1, CD279

Clone

29F.1A12

Uniprot

Q02242

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Antigen Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells

Immunogen

PD-1 cDNA followed by PD-1-Ig fusion protein

Aggregation

Aggregation level ≤ 5%

Related Antibody

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [29F.1A12] (ICH1091UL)

More Information

Bio X Cell:

ICH1091 is up to 19% cheaper for academia & non-profits and up to 47% cheaper for industry than the equivalent products BE0273 (low endotoxin) and up to 7% for academia & up to 39% cheaper for industry for the the ultra-low endotoxin version from Bio X Cell (BP0273).

Product Benefits:

ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is manufactured in a cGMP compliant facility. Click here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars. ichorbio: the best antibodies for in vivo research.

Size:

ichorbio's 29F.1A12 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-PD-1 29F.1A12 clone - please contact us for pricing.

Immunogen:

PD-1 cDNA followed by PD-1-Ig fusion protein

Concentration:

1.0 - 5.0 mg/ml

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Flow Cytometry, Western Blot, Blocking, IHC (Frozen)

Application Notes:

Use:

Products are for research use only.

Isotype Control:

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Antibodies against the same target:

Anti-PD-1 In Vivo Antibody - Low Endotoxin [RMP1-14] (ICH1132), Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)

Alternative Names: