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Mouse Anti-Mouse PD-1 (29F.1A12) Recombinant Antibody

Name: Mouse Anti-Mouse PD-1 (29F.1A12) Recombinant Antibody
SKU: ICH1211
Price: 315 USD
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$315.00 - $2,016.00

Mouse Anti-Mouse PD-1 (29F.1A12) Recombinant Antibody

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Species Reactivity

Mouse

Target

PD-1

Concentration

1.0 - 5.0 mg/ml

Isotype

Mouse IgG2a

Host

Mouse

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Application Notes

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.

Applications

Flow Cytometry, Western Blot, Blocking, IHC (Frozen)

Storage

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purification Method

This monoclonal antibody was purified using Protein A

Specificity

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Shipping Conditions

Blue ice

Background

PD-1 is an inhibitory checkpoint receptor on activated T cells that limits immune responses and is a cornerstone target of cancer immunotherapy research. Clone 29F.1A12 is a recombinant mouse IgG2a monoclonal antibody recognising mouse PD-1, validated for flow cytometry, Western blot, blocking, and frozen IHC. Anti-PD-1 29F.1A12 is supplied in a low-endotoxin format suitable for in vivo use and is manufactured in scalable milligram to gram quantities for bulk and high-throughput laboratories. As a non-therapeutic, research-use-only reagent, it offers consistent lot-to-lot reproducibility for mouse checkpoint-blockade studies.

Other Names

Programmed cell death protein 1, Pdcd1, CD279

Clone

29F.1A12

Uniprot

Q02242

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Antigen Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells

Immunogen

PD-1 cDNA followed by PD-1-Ig fusion protein