Anti-Mouse CD40 (FGK4.5) In Vivo Antibody - Low Endotoxin

Pircher, H. et al. MHC I tetramer staining tends to overestimate the number of functionally relevant self-reactive CD8 T cells in the preimmune repertoire. Eur. J. Immunol., 2023, 53: 2350402.

M. Stepanechko et al., "Single Treatment Boiling Histotripsy Focused Ultrasound Ablation Neither Negates nor Enhances the Activity of α-CD40 in a Pancreatic Cancer Model," in IEEE Transactions on Biomedical Engineering, 2025, 72, 11: 3404-3418.

Demir ZEF et al. Focused Ultrasound Thermal Ablation and CD40 Agonism Reprograms Breast Tumor Immunity to Drive Regression and Memory. bioRxiv, 2026, 2026.03.02.708396.

Mouse

CD40

>1mg/ml

Rat IgG2a

Rat

Anti-CD40 In Vivo Antibody - Ultra Low Endotoxin [FGK4.5] (ICH1073UL)

Bulk Anti-Human CD40 (G28.5) Antibody (ICH1156), Dacetuzumab Biosimilar - Research Grade (ICH5030), Sotigalimab Biosimilar - Research Grade (ICH5031), Selicrelumab Biosimilar - Research Grade (ICH5032), Lucatumumab Biosimilar - Research Grade (ICH5129), Abiprubart Biosimilar - Research Grade (ICH5323), Bleselumab Biosimilar - Research Grade (ICH5407), Cifurtilimab Biosimilar - Research Grade (ICH5437), Dalnicastobart Biosimilar - Research Grade (ICH5462), Giloralimab Biosimilar - Research Grade (ICH5571), Iscalimab Biosimilar - Research Grade (ICH5610), Mitazalimab Biosimilar - Research Grade (ICH5689), Ravagalimab Biosimilar - Research Grade (ICH5806), Teneliximab Biosimilar - Research Grade (ICH5915)

Aggregation level ≤ 5%

Recombinant Mouse CD40 Fusion Protein

B cells, basal epithelial cells, macrophages, follicular dendritic cells, and endothelial cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P27512

21939

Blue ice

This antibody targets mouse CD40 using clone FGK4.5, a rat IgG2a monoclonal raised against the mouse antigen. FGK4.5 is one of the most widely used agonistic anti-CD40 clones in immunology, valued for its ability to engage and activate CD40 signalling on antigen-presenting cells rather than simply mark the target. That functional agonist behaviour makes it well suited to in-vivo and mechanistic studies of dendritic cell and macrophage licensing, T-cell priming, and antitumour immunity, as well as to flow cytometry and western blot applications listed for the product. The preparation is manufactured for in-vivo work: low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg), supplied in bulk milligram-to-gram quantities, and provided as a research-use-only (RUO) reagent. The rat IgG2a format allows use with matched rat IgG2a isotype controls. Low endotoxin and bulk formatting reduce confounding innate immune activation and support the repeated dosing and larger cohorts typical of syngeneic tumour and immunisation models. CD40 (UniProt P27512) is a type I transmembrane glycoprotein of the tumour necrosis factor receptor (TNFR) superfamily. It is expressed on antigen-presenting cells including dendritic cells, macrophages, and B cells, as well as on some endothelial and epithelial cells. Its natural ligand is CD40L (CD154), expressed largely on activated CD4+ T cells and platelets. CD40-CD40L engagement is a central costimulatory axis: on dendritic cells it drives maturation, upregulation of MHC and costimulatory molecules, and cytokine production, thereby "licensing" APCs to prime CD8+ T-cell responses. On B cells it promotes proliferation, germinal centre formation, immunoglobulin class switching, and affinity maturation. Because CD40 stimulation can convert tolerogenic APCs into activating ones, agonism of this pathway is a major strategy for boosting antitumour and antiviral immunity.

B-cell surface antigen CD40, Bp50, CD40L receptor, Tumor necrosis factor receptor superfamily member 5, Tnfrsf5, FGK4 5, FGK4-5, FGK45

FGK4.5

Anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) recognizes an epitope on Mouse CD40

This monoclonal antibody was purified using Protein G

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added

anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Functional Assays, Flow Cytometry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0016-2, BP0016-2

Anti-Mouse CD40/Bp50 (FGK4.5) In Vivo Antibody - Low Endotoxin

Q: Is clone FGK4.5 suitable for in vivo use?

A: Yes — FGK4.5 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD40 studies as well as in vitro work.

Q: Can I order FGK4.5 in bulk or gram quantities?

A: Yes, this anti-mouse CD40 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone FGK4.5 target?

A: It is an anti-mouse CD40 monoclonal antibody that binds CD40 and is used in functional and blocking studies; for research use only (RUO).