Anti-Mouse CD209b (22D1) In Vivo Antibody - Low Endotoxin

Cao, Y. et al. Injectable hydrogel loaded with lysed OK-432 and doxorubicin for residual liver cancer after incomplete radiofrequency ablation. J Nanobiotechnol, 2023, 21, 404.

Mouse

CD209b

>1mg/ml

Armenian Hamster IgG1 Kappa

Armenian Hamster

Aggregation level ≤ 5%

Purified Recombinant Mouse CD209b (C-terminal peptide)

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Q8CJ91

69165

Blue ice

This is the widely used clone 22D1, an Armenian hamster IgG1 kappa monoclonal that recognizes mouse CD209b (SIGN-R1), a marginal-zone macrophage C-type lectin. It is offered as a low-endotoxin, in-vivo-ready preparation in bulk milligram-to-gram quantities for research use only, making it well suited to functional and in-vivo studies in mice where endotoxin contamination and formulation volume are limiting. Because 22D1 binds the carbohydrate-recognition region of SIGN-R1, it is frequently applied not only for detection (flow cytometry, frozen-section IHC, immunofluorescence, immunocytochemistry, Western blot, ELISA) but also as a blocking/functional reagent to interrupt SIGN-R1-mediated ligand capture in vivo and ex vivo. The hamster host and cross-reactivity profile make it a standard tool for labelling and functionally probing splenic marginal-zone and lymph-node medullary macrophage populations. As with all reagents supplied here, this content is guidance for ichorbio to review and the material is intended strictly for research, not diagnostic or therapeutic use. CD209b, also called SIGN-R1, is a type II transmembrane C-type lectin and one of the five mouse homologues of human DC-SIGN (CD209). It is expressed at high levels on splenic marginal-zone macrophages and lymph-node medullary macrophages. Through its calcium-dependent carbohydrate-recognition domain it binds high-mannose oligosaccharides and dextran, and it captures bacterial capsular polysaccharides such as those of Streptococcus pneumoniae, contributing to clearance of encapsulated organisms and to T-independent antibody responses. SIGN-R1 also participates in innate recognition and complement activation: it can bind C1q and promote classical complement C3 deposition on polysaccharide, and it has been reported to associate with pattern-recognition partners on the macrophage surface. Its restricted, well-defined expression makes it a valuable marker of marginal-zone macrophages.

DC-SIGN-related protein 1, DC-SIGNR1, OtB7

22D1

Anti-CD209b In Vivo Antibody - Low Endotoxin (22D1) recognizes an epitope on Mouse CD209b, also known as SIGN-R1

This monoclonal antibody was purified using Protein A

Phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no BSA sodium azide added.

anti-CD209b In Vivo Antibody - Low Endotoxin (22D1) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

IHC (Frozen), Flow Cytometry, Western Blot, Blocking, Immunofluorescence, ELISA, Immunocytochemistry

Blocking: Clone 22D1 has been reported to block CD209b in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0220

Anti-Mouse CD209b/DC-SIGNR1 (22D1) In Vivo Antibody - Low Endotoxin

Q: Is clone 22D1 suitable for in vivo use?

A: Yes — 22D1 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD209b studies as well as in vitro work.

Q: Can I order 22D1 in bulk or gram quantities?

A: Yes, this anti-mouse CD209b antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 22D1 target?

A: It is an anti-mouse CD209b monoclonal antibody that binds CD209b and is used in functional and blocking studies; for research use only (RUO).