Bulk Anti-Human CD40 (G28.5) Antibody

Human

CD40

>1mg/ml

Mouse IgG1 Kappa

Mouse

Anti-Mouse CD40 (FGK4.5) In Vivo Antibody - Low Endotoxin (ICH1073), Dacetuzumab Biosimilar - Research Grade (ICH5030), Sotigalimab Biosimilar - Research Grade (ICH5031), Selicrelumab Biosimilar - Research Grade (ICH5032), Lucatumumab Biosimilar - Research Grade (ICH5129), Abiprubart Biosimilar - Research Grade (ICH5323), Bleselumab Biosimilar - Research Grade (ICH5407), Cifurtilimab Biosimilar - Research Grade (ICH5437), Dalnicastobart Biosimilar - Research Grade (ICH5462), Giloralimab Biosimilar - Research Grade (ICH5571), Iscalimab Biosimilar - Research Grade (ICH5610), Mitazalimab Biosimilar - Research Grade (ICH5689), Ravagalimab Biosimilar - Research Grade (ICH5806), Teneliximab Biosimilar - Research Grade (ICH5915)

Aggregation level ≤ 5%

CD40 is widely expressed on antigen-presenting cells such as dendritic cells, B cells, macrophages, and monocytes, in addition to non-immune endothelial cells, basal epithelial cells, and a variety of tumors.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P25942

Blue ice

This is clone G28.5, a mouse IgG1 kappa monoclonal antibody directed against human CD40. G28.5 is a long-established, widely characterized anti-human CD40 clone that recognizes an epitope on the CD40 extracellular domain and has been used extensively to interrogate CD40 signaling on B cells, dendritic cells, and other antigen-presenting cells. Its human specificity and functional activity make it well suited to in-vitro functional assays, receptor-crosslinking and costimulation studies, and flow cytometry-based characterization of CD40-expressing populations. Supplied as a bulk, research-use-only reagent produced at scale (mg to gram quantities), it is intended for laboratories needing consistent, high-quantity material across large assay panels or process development. Low-endotoxin formulation reduces the risk of endotoxin-driven artifacts in sensitive cellular assays where nonspecific immune activation must be excluded. As a mouse IgG1 kappa, it offers relatively low intrinsic effector function, which can be advantageous when the goal is to study receptor engagement rather than Fc-mediated killing. RUO; not for diagnostic or therapeutic use. CD40 (P25942) is a type I transmembrane glycoprotein of the tumor necrosis factor receptor superfamily (TNFRSF5), constitutively expressed on B cells, dendritic cells, macrophages, and many non-hematopoietic cells. Its ligand, CD40L (CD154), is expressed predominantly on activated CD4+ T cells. CD40-CD40L engagement is a central costimulatory axis in adaptive immunity: on B cells it drives proliferation, germinal center formation, immunoglobulin class-switch recombination, and antibody affinity maturation; on dendritic cells and macrophages it promotes maturation, upregulation of costimulatory molecules, and proinflammatory cytokine production, thereby "licensing" APCs to prime effector T cells. Signaling proceeds through TRAF adaptor recruitment to the cytoplasmic tail, activating NF-kB, MAPK, and PI3K pathways. Loss-of-function mutations in CD40 or CD40L cause hyper-IgM syndrome. CD40 is also a therapeutic target of interest in oncology, where agonist antibodies aim to activate APCs and drive antitumor immunity.

Tumor necrosis factor receptor superfamily member 5, BP50, CD40L receptor, G28 5, G28-5, G285

G28.5

Clone G28.5 recognizes an epitope on human CD40

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Functional Assays, Flow Cytometry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone G28.5 suitable for in vivo CD40 studies?

A: Yes - G28.5 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD40 studies and in vitro work.

Q: Can I order anti-human CD40 clone G28.5 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone G28.5 target?

A: It is an anti-human CD40 monoclonal used to study CD40 signalling on B cells and antigen-presenting cells; for research use only.