Bulk Anti-Human CD11a (38) Antibody
Bulk anti-human CD11a antibody (38)
Product Benefits:ichorbio's anti-human CD11a antibody (38) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Target:CD11a
Clone:38
Isotype:Mouse IgG2a
Other Names:LFA-1 alpha, LFA-1A, Integrin alpha-L, ITGAL
Uniprot: Host:Mouse
Species Reactivity:Human
Specificity:Anti-human CD11a antibody (38) recognizes Human CD11a. Anti-Human CD11a (Anti-LFA-1a) recognizes the (Mr 175 kDa) a chain of the leukocyte function associated antigen-1 (LFA-1). The leukocyte integrin LFA-1 is a member of a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. LFA-1A binds to domain 1 of an intercellular adhesion molecule (ICAM) on target tissues.
Purification Method:This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:The CD11a antigen is present on most leukocytes and plays a vital role in cellular immune responses which require adhesion. Lymphocyte activation via the T-cell antigen receptor (TCR) up-regulates LFA-1 function. The absence of LFA-1A or B may induce the disease state known as leukocyte adhesion deficiency (LAD). Tumor cells which do not express LFA-1 may have avoided cellular immune-responses such as those carried out by natural killer cells (NK).
Background:Anti-CD11a may be used to study a variety of biological phenomena related to the function of cell adhesion molecules. This antibody may be used to study transplantation rejections and to assist in characterizing lymphomas and leukemias.
Immunogen:Unknown
Concentration:1.0 - 5.0 mg/ml
Formulation:0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation
Application Notes:Each investigator should determine their own optimal working dilution for specific applications.
Use:Products are for research use only.
Isotype Control:Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)
Antibodies against the same target:Anti-CD11a In Vivo Antibody - Low Endotoxin [FD441.8] (ICH1047), Anti-CD11a In Vivo Antibody - Low Endotoxin [I21/7] (ICH1048)
Concentration | 6.36mg/ml |
---|---|
isotype | Mouse IgG2a |
Host | Mouse |