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Bulk Anti-Human CD8 (UCHT-4) Antibody
$400.00 - $2,945.00
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Bulk Anti-Human CD8 (UCHT-4) Antibody
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Species Reactivity
Human
Target
CD8
Concentration
1.0 - 5.0 mg/ml
Isotype
Mouse IgG2a
Host
Mouse
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Applications
Flow Cytometry, Western Blot, Immunoprecipitation, Functional Assays, IHC, Immunofluorescence, ELISA
Storage
anti-CD8 In Vivo Antibody - Low Endotoxin (UCHT-4) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method
This monoclonal antibody was purified using Protein A
Specificity
Anti-CD8 In Vivo Antibody - Low Endotoxin (UCHT-4) recognizes Human CD8. Anti-Human CD8 recognizes a T-cell glycoprotein cell surface complex (Mr 32 kDa) which defines the cytotoxic/suppressor population of mature T-lymphocytes. The CD8 complex interacts with class I MHC molecules resulting in increased adhesion between CD8+ T-lymphocytes and their target cells. Binding of the CD8 antigen to Class I MHC molecules enhances T-cell activation. The cytoplasmic portion of the CD8 antigen is associated with a protein tyrosine kinase (p56lck).
Shipping Conditions
Blue ice
Background
CD8 marks cytotoxic T lymphocytes and serves as a co-receptor that stabilises engagement of MHC class I molecules during antigen recognition. Clone UCHT-4, a mouse IgG2a monoclonal antibody against human CD8, has been validated for flow cytometry, Western blot, immunoprecipitation, IHC, immunofluorescence, ELISA and functional assays. In vivo grade UCHT-4 is prepared in a low-endotoxin formulation and scaled from milligram (mg) to gram (g) quantities to meet the demands of bulk and high-throughput laboratories. Supplied for research use only (RUO) as a non-therapeutic reagent, it offers dependable performance for cytotoxic T-cell research.
Clone
UCHT-4
Uniprot
P01732
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Antigen Distribution
The CD8 antigen is expressed on approximately 19-48% of normal human peripheral blood lymphocytes and > 90% of normal thymocytes. It is also present on a subset of NK cells. Subjects infected with HIV have been shown to exhibit increased levels of CD8+ ly
Aggregation
Aggregation level ≤ 5%
Antibodies Against The Same Target
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