Anti-Mouse CD8a (2.43) In Vivo Antibody - Low Endotoxin

Tang, Dongyang et al. Augment proteasome inhibitor efficacy activates CD8+ T cell-mediated antitumor immunity in breast cancer. Cell Reports Medicine, 2025, Volume 6, Issue 7, 102211

Mouse

CD8a

>1mg/ml

Rat IgG2b

Rat

Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin [2.43] (ICH1045UL)

Anti-Mouse CD8 (YTS-169) In Vivo Antibody - Low Endotoxin (ICH1043), Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin (ICH1044), Anti-Mouse CD8 (116-13.1) In Vivo Antibody - Low Endotoxin (ICH1249)

Aggregation level ≤ 5%

CD8a (Ly 2.2) is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and prot

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01731

Blue ice

This antibody targets mouse CD8a (Lyt-2) using clone 2.43, a rat IgG2b monoclonal raised against mouse CD8-bearing cells and one of the most widely cited clones for in vivo CD8 T-cell depletion. The rat IgG2b isotype supports Fc-dependent effector clearance of CD8+ T cells in immunocompetent mice, making the clone well suited to functional and mechanistic studies rather than staining alone. It is validated across immunoprecipitation, Western blot, flow cytometry, immunofluorescence and functional assays, giving researchers a single reagent for both target detection and in vivo intervention. ichor.bio supplies 2.43 in an in-vivo-ready, low-endotoxin format (research grade <1 EU/mg; ultra-low <0.5 EU/mg) at bulk mg-to-gram scale, reducing endotoxin-driven confounders in immunology, tumour immunology and infection models. All material is for research use only (RUO). Because researchers typically search by clone, this product is listed under the 2.43 designation to match established literature and prior in vivo depletion studies. CD8a (Lyt-2, encoded by Cd8a) is a transmembrane glycoprotein of the immunoglobulin superfamily that serves as the defining co-receptor of cytotoxic T lymphocytes. On most conventional CD8+ T cells it is expressed as a CD8 alpha-beta heterodimer, while a CD8 alpha-alpha homodimer appears on subsets of intraepithelial lymphocytes, NK cells and dendritic-cell populations. CD8 binds the alpha-3 domain of MHC class I molecules, stabilising the interaction between the T-cell receptor and peptide-MHC-I complexes and recruiting the kinase Lck to the intracellular signalling machinery. This co-receptor function lowers the threshold for antigen recognition and is essential for the priming, expansion and effector activity of cytotoxic T cells that eliminate virally infected and tumour cells. Because CD8a marks essentially all cytotoxic T cells, it is a primary lineage marker and a key handle for both identifying and functionally manipulating this population.

CD8 alpha chain, Lyt-2, Lyt2, 2 43, 2-43, 243

2.43

Anti-CD8a In Vivo Antibody - Low Endotoxin (2.43) recognizes an epitope on Mouse CD8a

This monoclonal antibody was purified using Protein G

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

anti-CD8a In Vivo Antibody - Low Endotoxin (2.43) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Immunoprecipitation, Western Blot, Flow Cytometry, Immunofluorescence, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 2.43 suitable for in vivo use?

A: Yes — 2.43 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD8a studies as well as in vitro work.

Q: Can I order 2.43 in bulk or gram quantities?

A: Yes, this anti-mouse CD8a antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 2.43 target?

A: It is an anti-mouse CD8a monoclonal antibody that binds CD8a and is used in functional and blocking studies; for research use only (RUO).