Back to Top
Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin
Add recommended extras
Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Rat IgG1 |
| Host | Rat |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation |
| Storage | anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycle |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein G |
| Specificity | Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1 |
| Species Reactivity | Mouse |
| Target | CD8 beta |
| Shipping Conditions | Blue ice |
| background | CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes. |
| Other names | Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3 |
| clone | 53-5.8 |
| UniProt | P10300 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | Majority of thymocytes, cytotoxic T cell subsets |
| Immunogen | Mouse thymus or spleen |
| Aggregation | Aggregation level ≤ 5% |
| Related Antibody | Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin [53-5.8] (ICH1046UL) |
Write Your Own Review