Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

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ICH1046
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Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

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Concentration 1.0 - 5.0 mg/ml
Isotype Rat IgG1
Host Rat
Use Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes Each investigator should determine their own optimal working dilution for specific applications.
Applications Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation
Storage anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycle
Endotoxin ≤ 1.0 EU/mg as determined by the LAL method
Purity >95% by SDS-PAGE and HPLC
Formulation 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method This monoclonal antibody was purified using Protein G
Specificity Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1
Species Reactivity Mouse
Target CD8 beta
Shipping Conditions Blue ice
background CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.
Other names Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3
clone 53-5.8
UniProt P10300
Buffer ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution Majority of thymocytes, cytotoxic T cell subsets
Immunogen Mouse thymus or spleen
Aggregation Aggregation level ≤ 5%
Related Antibody Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin [53-5.8] (ICH1046UL)
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