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Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin
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Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Rat IgG2a Kappa |
| Host | Rat |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins. Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen) |
| Storage | anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein G |
| Specificity | Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant. |
| Species Reactivity | Mouse |
| Target | CD8a |
| Shipping Conditions | Blue ice |
| background | CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and _ chains that form the A(CD8a)/_(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/_ heterodimer is expressed on the surface |
| Other names | CD8 alpha chain, Lyt-2, Lyt2 |
| clone | 53-6.7 |
| UniProt | P01731 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kina |
| Immunogen | Mouse thymus or spleen |
| Aggregation | Aggregation level ≤ 5% |
| Antibodies against the same target | Anti-CD8a In Vivo Antibody - Low Endotoxin [2.43] (ICH1045), Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin [2.43] (ICH1045UL) |
| Related Antibody | Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin [53-6.7] (ICH1044UL) |
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