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Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin
$400.00 - $3,780.00
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Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Species Reactivity
Mouse
Target
CD8a
Concentration
1.0 - 5.0 mg/ml
Isotype
Rat IgG2a Kappa
Host
Rat
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes
Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins.
Each investigator should determine their own optimal working dilution for specific applications.
Applications
Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)
Storage
anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method
This monoclonal antibody was purified using Protein G
Specificity
Anti-CD8a In Vivo Antibody - Ultra Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Shipping Conditions
Blue ice
Background
CD8a is the alpha chain of the CD8 co-receptor on cytotoxic T cells and a defining marker of the CD8 lineage. Clone 53-6.7 is a rat IgG2a kappa monoclonal antibody against mouse CD8a, validated across immunoprecipitation, Western blot, flow cytometry, CyTOF and frozen IHC. Anti-mouse CD8a 53-6.7 is supplied in a low-endotoxin format suitable for in vivo use and produced in scalable milligram (mg) to gram (g) quantities for bulk and high-throughput laboratories. It is a non-therapeutic, research-use-only (RUO) reagent offering consistent lot-to-lot reproducibility for mouse cytotoxic T-cell studies.
Other Names
CD8 alpha chain, Lyt-2, Lyt2
Clone
53-6.7
Uniprot
P01731
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Antigen Distribution
Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kina
Immunogen
Mouse thymus or spleen
Aggregation
Aggregation level ≤ 5%
Antibodies Against The Same Target
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