Rat IgG2b (1-2) In Vivo Isotype Control - Low Endotoxin

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SKU
ICH2243
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$129.00 - $23,460.00

Rat IgG2b (1-2) In Vivo Isotype Control - Low Endotoxin

Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Target
Trinitrophenol + KLH
Concentration
1.0 - 5.0 mg/ml
Isotype
IgG2b
Host
Rat
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes
This isotype control antibody should be used at the same concentration as the primary antibody.
Storage
This Low Endotoxin Functional Formulation conjugate is stable when stored at 2-8°C. Do not freeze.
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Specificity
This Rat IgG2b κ isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross-reactivity.
Shipping Conditions
Blue ice
Background
This rat IgG2b antibody, clone 1-2, is a matched isotype control with no relevant specificity in mouse systems, raised against an irrelevant hapten-carrier immunogen so it shares the format of rat IgG2b experimental antibodies without engaging biological targets. It is intended to establish background signal, define gating boundaries, and serve as a negative control in functional and in vivo studies. Supplied in a low-endotoxin format suitable for in vivo use and manufactured in scalable milligram to gram quantities, it meets the needs of bulk and high-throughput laboratories. The reagent is for research use only and is entirely non-therapeutic.
Clone
1-2
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Aggregation
Aggregation level ≤ 5%
More Information
Product Benefits:

ichorbio's Rat IgG2b Isotype Control for In Vivo - Low Endotoxin (1-2) is manufactured in an cGMP compliant facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin [<0.5EU/mg] at an even higher purity [98% versus 95%]. ichorbio: the best antibodies for in vivo research.

Target:

Trinitrophenol and KLH.

Clone:

1-2

Isotype:

IgG2b

Host:

Rat

Specificity:

This Rat IgG2b isotype control antibody [Anti-KLH+Trinitrophenol] has been tested against selected species' cells and tissues to assure minimal cross reactivity.

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Concentration:

1.0 - 5.0 mg/ml

Formulation:

This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline [PBS] pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Pneumonia virus of mice Kilham's Rat Virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Mouse adenovirus Reovirus 3 Rat theilovirus Seoul virus

Storage:

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:

This Rat IgG2b isotype control antibody should be used at the same concentration as the primary antibody.

Use:

Products are for research use only.

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