Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin

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SKU
ICH1041
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$150.00

Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin

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Species Reactivity Mouse
Target CD3e
Concentration 1.0 mg/ml
Isotype Armenian Hamster IgG
Host Armenian Hamster
Use Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications.
Applications IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking
Storage anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin ≤ 1.0 EU/mg as determined by the LAL method
Purity >95% by SDS-PAGE and HPLC
Formulation 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method This monoclonal antibody was purified using Protein A
Specificity Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
Shipping Conditions Blue ice
background CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell a
Other names T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
clone 145-2C11
UniProt P22646
Buffer ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
Immunogen H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Aggregation Aggregation level ≤ 5%
Antibodies against the same target Anti-CD3e In Vivo Antibody - Low Endotoxin [500A2] (ICH1040), Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [500A2] (ICH1040UL)
Related Antibody Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [145-2C11] (ICH1041UL)
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