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Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin
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Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin
| Species Reactivity | Mouse |
|---|---|
| Target | CD3e |
| Concentration | 1.0 mg/ml |
| Isotype | Armenian Hamster IgG |
| Host | Armenian Hamster |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking |
| Storage | anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e |
| Shipping Conditions | Blue ice |
| background | CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell a |
| Other names | T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e |
| clone | 145-2C11 |
| UniProt | P22646 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested. |
| Immunogen | H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37 |
| Aggregation | Aggregation level ≤ 5% |
| Antibodies against the same target | Anti-CD3e In Vivo Antibody - Low Endotoxin [500A2] (ICH1040), Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [500A2] (ICH1040UL) |
| Related Antibody | Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [145-2C11] (ICH1041UL) |
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