Anti-Mouse LPAM1 (DATK32) In Vivo Antibody - Low Endotoxin

Mouse

LPAM1

>1mg/ml

Rat IgG2a

Rat

Aggregation level ≤ 5%

TK1 cells

A majority of peripheral lymphocytes and subsets of bone marrow cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

RRID:AB_2921485

Blue ice

Clone DATK32 is a rat IgG2a monoclonal antibody raised against mouse LPAM1, the integrin alpha4beta7 (also called integrin alpha-4/beta-7 heterodimer). It recognizes the combinatorial alpha4beta7 epitope rather than the individual alpha4 or beta7 subunits, making it a specific reporter and functional modulator of the intact gut-homing integrin. This lot is offered as a low-endotoxin, RUO-grade preparation suited to in-vivo and functional work: it is validated for Western Blot and blocking applications, and its capacity to interfere with alpha4beta7-dependent adhesion makes it a common tool for interrogating lymphocyte trafficking to intestinal tissue. The rat IgG2a format on a mouse target allows straightforward pairing with appropriate isotype controls and secondary reagents. Low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) reduces the risk of confounding innate-immune activation in animal experiments, and bulk milligram-to-gram scale supports multi-cohort in-vivo dosing studies. All material is research-use-only and intended as guidance for the investigator to validate in their own system. LPAM1 (lymphocyte Peyer's patch adhesion molecule 1) is the integrin alpha4beta7 heterodimer, formed by the alpha4 (ITGA4/CD49d) and beta7 (ITGB7) subunits. It is expressed on subsets of lymphocytes, including memory/effector T cells, B cells, and innate lymphoid cells, and serves as the principal gut-homing receptor. Its main counter-receptor is MAdCAM-1 (mucosal addressin cell adhesion molecule-1), which is displayed on high endothelial venules of Peyer's patches and mesenteric lymph nodes and on lamina propria venules. Alpha4beta7 also binds VCAM-1 and fibronectin with lower avidity. Engagement of MAdCAM-1 mediates rolling and firm arrest of circulating lymphocytes, directing their entry into gut-associated lymphoid tissue and intestinal mucosa. This axis is central to mucosal immune surveillance and to the pathogenesis of inflammatory bowel disease, making alpha4beta7 a validated therapeutic target for blockade.

Integrin alpha 4 beta 7, CD49d/beta7, ITGA4, ITGB7

DATK32

This Rat anti-Mouse LPAM-1 Clone DATK32 antibody recognizes an epitope on Mouse LPAM1 a 130 kDa integrin B7 chain associates with the 150 kDa integrin A4 (CD49d) chain to form LPAM-1, a member of the Ig superfamily

This monoclonal antibody was purified using Protein G

PBS pH 7.4, 150 mM NaCl, without sodium azide added as a preservative.

Store this antibody at 2-8°C. Do not freeze.

Western Blot, Blocking

Blocking: Clone DATK32 antibody has been reported to block LPAM-1-mediated cell adhesion in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0034

Anti-Mouse LPAM1/CD49d/beta7 (DATK32) In Vivo Antibody - Low Endotoxin

Q: Is clone DATK32 suitable for in vivo use?

A: Yes — DATK32 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo LPAM1 studies as well as in vitro work.

Q: Can I order DATK32 in bulk or gram quantities?

A: Yes, this anti-mouse LPAM1 antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone DATK32 target?

A: It is an anti-mouse LPAM1 monoclonal antibody that binds LPAM1 and is used in functional and blocking studies; for research use only (RUO).