Anti-Mouse LPAM1 (DATK32) In Vivo Antibody - Low Endotoxin

Bulk anti-LPAM1 In Vivo Antibody - Low Endotoxin (DATK32)

Product Benefits:

ichorbio's anti-LPAM1 In Vivo Antibody - Low Endotoxin (DATK32) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

LPAM1

Clone:

DATK32

Isotype:

Rat IgG2a

Other Names:

Integrin alpha 4 beta 7, CD49d/beta7, ITGA4, ITGB7

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-LPAM1 In Vivo Antibody - Low Endotoxin (DATK32) recognizes an epitope on Mouse LPAM1 a 130 kDa integrin B7 chain associates with the 150 kDa integrin A4 (CD49d) chain to form LPAM-1, a member of the Ig superfamily

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

A majority of peripheral lymphocytes and subsets of bone marrow cells

Immunogen:

TK1 cells

Concentration:

≥ 2.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Blocking

Application Notes:

Blocking: Clone DATK32 antibody has been reported to block LPAM-1-mediated cell adhesion in vivo. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)

Alternative Names:

• Antigen CD49D antibody
• CD49d antibody
• IA4 antibody
• Integrin alpha 4 subunit antibody
• Integrin alpha IV antibody
• Integrin beta 7 antibody
• Integrin beta 7 subunit antibody
• ITGA4 antibody
• ITGB7 antibody
• LPAM1 antibody