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Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Low Endotoxin

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SKU

ICH1110

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$390.00

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Bulk anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7)

Product Specific Citations:

Mine et al. TYK2 signaling promotes the development of autoreactive CD8+ cytotoxic T lymphocytes and type 1 diabetes. Nature Communications volume 15, Article number: 1337 (2024)

Bio X Cell:

ICH1110 is up to 37% cheaper for industry than the equivalent product BE0102 from Bio X Cell.

Product Benefits:

ichorbio's anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) is manufactured in a cGMP compliant. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

IFN beta

Clone:

HDB-4A7

Size:

ichorbio's HDB-4A7 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-interferon beta HDB-4A7 clone - please contact us for pricing.

Isotype:

Mouse IgG2a

Other Names:

IFN-b, IFN-beta, Type-1 interferon, Interferon beta

Host:

Mouse

Species Reactivity:

Mouse

Specificity:

Anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

Purification Method:

This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Immunogen:

Plasmid DNA encoding murine IFNb

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Blocking, Functional Assays

Application Notes:

Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)

Antibodies against the same target:

Anti-IFN beta In Vivo Antibody - Low Endotoxin [MIB-5E9.1] (ICH1124)

Bulk anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7)

More Information
Product Specific Citations	Mine et al. TYK2 signaling promotes the development of autoreactive CD8+ cytotoxic T lymphocytes and type 1 diabetes. Nature Communications volume 15, Article number: 1337 (2024)
Concentration	1.0 - 5.0 mg/ml
Isotype	Mouse IgG2a
Host	Mouse
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days. Each investigator should determine their
Applications	Western Blot, Blocking, Functional Assays
Storage	anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. Do not freeze. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and HPLC
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method	This monoclonal antibody was purified using Protein A
Specificity	Anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and n
Species Reactivity	Mouse
Target	IFN beta
Shipping Conditions	Blue ice
Other names	IFN-b, IFN-beta, Type-1 interferon, Interferon beta
clone	HDB-4A7
UniProt	P01575
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Immunogen	Plasmid DNA encoding murine IFNb
Aggregation	Aggregation level ≤ 5%
Antibodies against the same target	Anti-IFN beta In Vivo Antibody - Low Endotoxin [MIB-5E9.1] (ICH1124)
Related Antibody	Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin [HDB-4A7] (ICH1110UL)