Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin

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ICH1130

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Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin

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Concentration	0.5 mg/ml
Isotype	Rat IgG2a
Host	Rat
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity. Each investigator should determine their own optimal working dilution for specific applications.
Applications	Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA
Storage	Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at ï؟½80°C in a manual defrost freezer. Avoid Repeated Freez
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and HPLC
Formulation	No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Purification Method	This monoclonal antibody was purified using Protein G
Specificity	Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.
Species Reactivity	Mouse, Human
Target	CD45R
Shipping Conditions	Blue ice
background	CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.
Other names	Leukocyte common antigen, L-CA, T200, CD45, Receptor-type tyrosine-protein phosphatase C, PTPRC, B220
clone	RA3-6B2
UniProt	P08575
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .
Immunogen	Abelson murine leukemia virus-induced pre-B tumor cells
Aggregation	Aggregation level ≤ 5%
Antibodies against the same target	Anti-CD45 In Vivo Antibody - Low Endotoxin [I3/2.3] (ICH1057)
Related Antibody	Anti-CD45R In Vivo Antibody - Ultra Low Endotoxin [RA3-6B2] (ICH1130UL)