Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin

Mouse

NKG2A/C/E

>1mg/ml

Rat IgG2a

Rat

Aggregation level ≤ 5%

CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94

NK cells and NK T cells from C57BL/6 mouse

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Q9Z202

Blue ice

This product is the rat anti-mouse NKG2A/C/E monoclonal antibody, clone 20D5 (rat IgG2a isotype), supplied in an in-vivo-ready, low-endotoxin format for research use only. Clone 20D5 recognizes an epitope shared across the mouse CD94-associated NKG2 family members NKG2A, NKG2C and NKG2E (CD159a/c/e), receptors expressed predominantly on natural killer (NK) cells and subsets of activated CD8 T cells. Because 20D5 binds the shared NKG2 ectodomain, it is widely used to interrogate and modulate NKG2A-mediated inhibitory signaling in NK-cell biology, tumor immunology, and viral immunity. The rat IgG2a backbone and validated cross-reactivity within the mouse NKG2A/C/E family make it well suited to functional flow-cytometry phenotyping and in-vivo receptor-blockade experiments in immunocompetent mouse models. ichorbio supplies this clone at low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and in bulk milligram-to-gram quantities to support repeat-dosing in-vivo study designs, giving researchers a consistent, affordable reagent for NK-cell checkpoint and adoptive-transfer work. All material is intended as a research reagent for the customer to validate in their own system. NKG2A, NKG2C and NKG2E are C-type lectin-like receptors that pair covalently with CD94 to form heterodimers on NK cells and some activated CD8 T cells. In mice these receptors recognize the non-classical MHC class I molecule Qa-1b (the functional homolog of human HLA-E), which presents peptides derived from the leader sequences of classical MHC class I. The CD94/NKG2A heterodimer is inhibitory: engagement of Qa-1b delivers an ITIM-mediated "off" signal that restrains NK-cell cytotoxicity and cytokine output, contributing to missing-self recognition and self-tolerance. In contrast, CD94/NKG2C and NKG2E are activating receptors that signal through DAP12. Because NKG2A functions as an NK-cell checkpoint that tumors and virally infected cells can exploit by upregulating Qa-1b/HLA-E, blocking this inhibitory axis is of substantial interest for enhancing NK- and T-cell-mediated immunity.

CD159a, CD159c, CD159e, NKG2C, NKG2E

20D5

Clone 20D5 antibody reacts with mouse NKG2A, NKG2C, and NKG2E however, this hybridoma has not been epitope mapped to identify the binding site.

This monoclonal antibody was purified using Protein G

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry

For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 20D5 suitable for in vivo NKG2A/C/E studies?

A: Yes - 20D5 is a low-endotoxin, in vivo-grade antibody suitable for in vivo NKG2A/C/E studies and in vitro work.

Q: Can I order anti-mouse NKG2A/C/E clone 20D5 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone 20D5 target?

A: It is an anti-mouse monoclonal recognising NKG2A/C/E receptors on NK cells and some T cells; for research use only.