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Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin
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Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Rat IgG2a |
| Host | Rat |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | For flow cytometric staining, the suggested use of this reagent is ï؟½0.5 µg per million cells in 100 ï؟½l volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed. |
| Applications | Flow Cytometry |
| Storage | This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | <1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein G |
| Specificity | Clone 20D5 antibody reacts with mouse NKG2A, NKG2C, and NKG2E however, this hybridoma has not been epitope mapped to identify the binding site. |
| Species Reactivity | Mouse |
| Target | NKG2A/C/E |
| Shipping Conditions | Blue ice |
| Other names | CD159a, CD159c, CD159e, NKG2C, NKG2E |
| clone | 20D5 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | NK cells and NK T cells from C57BL/6 mouse |
| Immunogen | CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94 |
| Aggregation | Aggregation level ≤ 5% |
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