Anti-Mouse 4-1BBL (TKS-1) In Vivo Antibody - Low Endotoxin

Mouse

4-1BBL

>1mg/ml

Rat IgG2a Kappa

Rat

Aggregation level ≤ 5%

BALB/c mouse B lymphoma line 2PK-3

4-1BBL is expressed by activated B cells, macrophages, and dendritic cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P41274

Blue ice

This antibody targets mouse 4-1BBL (also known as TNFSF9 or CD137L; UniProt P41274) and is derived from the rat clone TKS-1, a rat IgG2a kappa monoclonal raised against the mouse antigen. The rat IgG2a isotype and validated blocking activity make this clone well suited to in-vivo and functional immunology studies in mouse models, where it is used to interrupt 4-1BBL/4-1BB co-stimulatory signalling and to interrogate T-cell and NK-cell responses. Reported applications include blocking, functional assays, and Western blot. The preparation is manufactured at low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) to minimise confounding innate stimulation during in-vivo dosing, and is supplied in bulk milligram-to-gram quantities suitable for repeat-dosing tumour-immunology, infection, and autoimmunity cohorts. All material is for research use only (RUO). Researchers typically identify this reagent by its clone designation, TKS-1, when selecting a functional-grade anti-4-1BBL for co-stimulation-blockade experiments. 4-1BBL (TNFSF9 / CD137L) is a member of the tumour necrosis factor (TNF) superfamily and serves as the ligand for the co-stimulatory receptor 4-1BB (CD137, TNFRSF9). It is expressed on activated antigen-presenting cells including dendritic cells, macrophages, and B cells. Engagement of 4-1BB on activated CD8+ and CD4+ T cells and on NK cells delivers a co-stimulatory signal that promotes clonal expansion, effector cytokine production, cytotoxicity, and survival, in part through TRAF-dependent NF-kB and downstream survival-gene programmes. The 4-1BBL/4-1BB axis also supports memory T-cell maintenance and can act bidirectionally, signalling back into the ligand-bearing APC. Because this pathway amplifies antitumour and antiviral effector responses, it is a focus in tumour immunology, chronic infection, and autoimmunity research, where reagents that block or modulate the interaction are used to dissect its contribution.

CD137L, TNFSF9, 4-1BB Ligand, TKS-1, TKS 1, TKS1

TKS-1

TKS-1 activity is directed against 4-1BBL (CD137L)

This monoclonal antibody was purified using Protein G

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Blocking, Functional Assays, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone TKS-1 suitable for in vivo 4-1BBL studies?

A: Yes - TKS-1 is a low-endotoxin, in vivo-grade antibody suitable for in vivo 4-1BBL blocking and in vitro work.

Q: Can I order anti-mouse 4-1BBL clone TKS-1 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone TKS-1 target?

A: It is an anti-mouse 4-1BBL monoclonal used to study and block 4-1BB/4-1BBL costimulatory signalling; for research use only.