Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin

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ICH1055

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Anti-Mouse CD28 (37.51) In Vivo Antibody - Low Endotoxin

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Concentration	1.0 - 5.0 mg/ml
Isotype	Syrian Hamster IgG
Host	Syrian Hamster
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Functional Assay: Anti-Mouse CD28 (clone 37.51) has been reported in costimulation of T cells In vitro and In vivo. Each investigator should determine their own optimal working dilution for specific applications.
Applications	Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, IHC (Frozen)
Storage	anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and HPLC
Formulation	20 mM Histidine pH 6.0 containing 150 mM NaCl. NOTE: A buffer exchange into PBS for conjugation and MES for functional assays may be required depending on your application.
Purification Method	This monoclonal antibody was purified using Protein A
Specificity	Anti-CD28 In Vivo Antibody - Low Endotoxin (37.51) recognizes the CD28 co-stimulatory receptor, a ligand of CD80 (B7-1) and CD86 (B7-2). CD28 is important for transmitting co-stimulatory signals for optimal T-cell activation.
Species Reactivity	Mouse
Target	CD28
Shipping Conditions	Blue ice
background	CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is composed of a single Ig V-like extracellular domain, a transmembrane domain and an int
clone	37.51
UniProt	P31041
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	Anti-CD28 is present on most thymocytes, CD4+, CD8+ T-cells. The 37.51 antibody augments proliferation and IL-2 production by activated T-cells. Clone 37.51 antibody has been shown to stimulate the proliferation and cytokine production by activated T and
Immunogen	C57BL/6 mouse T-cell lymphoma EL-4
Aggregation	Aggregation level ≤ 5%
Antibodies against the same target	Anti-CD28 In Vivo Antibody - Low Endotoxin [CD28.2] (ICH1013)
Related Antibody	Anti-CD28 In Vivo Antibody - Ultra Low Endotoxin [37.51] (ICH1055UL)