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Anti-Mouse CD25 (PC61) In Vivo Antibody - Low Endotoxin
$400.00 - $3,780.00
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Anti-Mouse CD25 (PC61) In Vivo Antibody - Low Endotoxin
| Species Reactivity | Mouse |
|---|---|
| Target | IL2RA |
| Concentration | ≤ 2.0 mg/ml |
| Isotype | Rat IgG1 Lambda |
| Host | Rat |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Immunoprecipitation, Western Blot, Functional Assays, Blocking, Flow Cytometry, IHC (Frozen) |
| Storage | anti-CD25 In Vivo Antibody - Low Endotoxin (PC61) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein G |
| Specificity | Anti-CD25 In Vivo Antibody - Low Endotoxin (PC61) recognizes an epitope on Mouse IL-2 RA |
| Shipping Conditions | Blue ice |
| background | IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation. IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon act |
| Other names | Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, Il2ra, p55, CD25 |
| clone | PC61 |
| UniProt | P01590 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | The CD25 antigen is detected on CD4 positive T-cells but not on CD8 positive T-cells. However, when stimulated with phytohemagglutinin (PHA), the CD25 antigen can be detected on CD8 positive T cells. The CD25 antigen is also expressed on CD20 positive B c |
| Immunogen | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Aggregation | Aggregation level ≤ 5% |
| Antibodies against the same target | Anti-CD252 In Vivo Antibody - Low Endotoxin [RM134L] (ICH1079) |
| Related Antibody | Anti-CD25 In Vivo Antibody - Ultra Low Endotoxin [PC61] (ICH1054UL) |
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