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Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin
$150.00 - $23,460.00
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Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Species Reactivity
Mouse
Target
CD32/CD16
Concentration
0.5 mg/ml
Isotype
Rat IgG2b
Host
Rat
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Applications
Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX
Storage
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method
This monoclonal antibody was purified using Protein G
Specificity
Anti-CD32/CD16 In Vivo Antibody - Ultra-Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Shipping Conditions
Blue ice
Background
CD32/CD16 are Fc-gamma receptors (FcγRII/III) expressed on myeloid and other immune cells, and they are routinely blocked to prevent nonspecific antibody binding in immunoassays. Clone 2.4G2 is a rat IgG2b monoclonal antibody to mouse CD32/CD16, the standard Fc-block reagent, validated for Western blot, blocking, flow cytometry, frozen IHC, immunoprecipitation and CODEX. Anti-mouse CD32/CD16 2.4G2 is supplied low in endotoxin and suitable for in vivo use, with scalable milligram (mg) to gram (g) production for bulk laboratories. This non-therapeutic, research-use-only (RUO) reagent ensures consistent, reproducible Fc blocking in mouse immunology.
Other Names
Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII
Clone
2.4G2
Uniprot
P08508
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Antigen Distribution
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
Aggregation
Aggregation level ≤ 5%
Antibodies Against The Same Target
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Rat IgG2b (1-2) In Vivo Isotype Control - Low Endotoxin$129.00 - $23,460.00 -
In Vivo Buffer pH7.2$112.50 -
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