Anti-CD11C (N418) In Vivo Antibody - Low Endotoxin

Mouse

CD11c

>1mg/ml

Armenian Hamster IgG

Armenian Hamster

Bulk Anti-Human CD11c (3.9) Antibody (ICH1008)

Aggregation level ≤ 5%

Mouse spleen dendritic cells

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Q9QXH4

16411

Blue ice

This is the N418 clone, an Armenian hamster IgG raised against mouse CD11c (integrin alpha-X, UniProt Q9QXH4) and produced in Armenian hamster host. N418 is one of the most widely used and best-characterised anti-mouse CD11c antibodies, valued as a defining marker for dendritic cells and validated across Flow Cytometry, Western Blot, Immunoprecipitation, IHC (Frozen), Immunofluorescence, Immunocytochemistry and ELISA. This preparation is manufactured for in vivo and functional immunology research: it is supplied at low endotoxin (research grade typically less than 1 EU/mg, with ultra-low-endotoxin options at less than 0.5 EU/mg) to limit confounding innate immune activation, and is available in bulk milligram-to-gram quantities suitable for multi-cohort mouse studies and repeat dosing. The antibody is provided for Research Use Only. Its hamster-anti-mouse format makes it appropriate for studies of dendritic cell biology, antigen delivery to DCs, and myeloid-lineage identification in mouse tumour, infection, autoimmunity and vaccine models where reagent purity and consistency across large in vivo experiments matter. CD11c (integrin alpha-X, ITGAX) is a type I transmembrane alpha integrin subunit that pairs non-covalently with the beta-2 integrin CD18 (ITGB2) to form the complement receptor 4 (CR4, alpha-X-beta-2) heterodimer. It binds ligands including the complement fragment iC3b, ICAM-1, fibrinogen and denatured proteins, contributing to leukocyte adhesion, phagocytosis and cell-cell interactions. In the mouse, CD11c is expressed most highly on conventional dendritic cells, where it serves as the canonical lineage-identifying marker, and is also found at lower levels on subsets of macrophages, monocytes, NK cells and some activated lymphocytes. Because of its strong association with dendritic cells, CD11c is central to studies of antigen presentation, T-cell priming and the initiation of adaptive immunity, and CD11c-driven systems are widely used to identify, image and manipulate DC populations in immunological research.

Integrin alpha-X, Itgax, Leukocyte adhesion glycoprotein p150, 95 alpha chain, Leukocyte adhesion receptor p150, 95

N418

Anti-CD11C In Vivo Antibody - Low Endotoxin (N418) recognizes an epitope on Mouse CD11C

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

anti-CD11C In Vivo Antibody - Low Endotoxin (N418) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunofluorescence, Immunocytochemistry, ELISA

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0038

Anti-CD11C (N418) In Vivo Antibody - Low Endotoxin

Q: Is clone N418 suitable for in vivo use?

A: Yes — N418 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo CD11c studies as well as in vitro work.

Q: Can I order N418 in bulk or gram quantities?

A: Yes, this anti-mouse CD11c antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone N418 target?

A: It is an anti-mouse CD11c monoclonal antibody that binds CD11c and is used in functional and blocking studies; for research use only (RUO).