Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody, Low Endotoxin

Bulk anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4)

Bio X Cell:

ICH1059 is up to 37% cheaper for industry than the equivalent product BE0020-1 from Bio X Cell. Product Benefits:

ichorbio's anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD54

Clone:

YN1/1.7.4

Size:

ichorbio's YN1/1.7.4 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CD54 YN1/1.7.4 clone - please contact us for pricing.

Isotype:

Rat IgG2b

Other Names:

Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10

Uniprot:

P13597

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) recognizes an epitope on Mouse ICAM-1

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.

Background:

Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.

Immunogen:

Mouse NS-1 cells

Concentration:

1.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Alternative Names: