Anti-Mouse CD45 (I3/2.3) In Vivo Antibody - Low Endotoxin

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ICH1057

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Anti-Mouse CD45 (I3/2.3) In Vivo Antibody - Low Endotoxin

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Concentration	1.0 - 5.0 mg/ml
Isotype	Rat IgG2b
Host	Rat
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ï؟½0.125 µg per million cells in 100 ï؟½l volume. Each investigator should determine their own optimal working dilution for specific applications.
Applications	Western Blot, Flow Cytometry, IHC (Frozen), IHC (Paraffin)
Storage	anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and HPLC
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method	This monoclonal antibody was purified using Protein G
Specificity	Anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) recognizes an epitope on Mouse CD45
Species Reactivity	Mouse
Target	CD45
Shipping Conditions	Blue ice
background	CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA). CD45 plays a key role in TCR and BCR signal transduction.
Other names	Receptor-type tyrosine-protein phosphatase C, Ptprc, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, LCA
clone	I3/2.3
UniProt	P06800
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	The CD45 antigen is present on all cells of hematopoietic origin, but not erythrocytes
Immunogen	Mouse lymphoma cell line
Aggregation	Aggregation level ≤ 5%
Antibodies against the same target	Rat anti Mouse/Human CD45R In Vivo Antibody - Low Endotoxin [RA3-6B2] (ICH1130), Rat anti Mouse/Human CD45R In Vivo Antibody - Ultra Low Endotoxin [RA3-6B2] (ICH1130UL)