Anti-Mouse CD45 (I3/2.3) In Vivo Antibody, Low Endotoxin

Bulk anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3)

Product Benefits:

ichorbio's anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD45

Clone:

I3/2.3

Isotype:

Rat IgG2b

Other Names:

Receptor-type tyrosine-protein phosphatase C, Ptprc, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, LCA

Uniprot:

P06800

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) recognizes an epitope on Mouse CD45

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The CD45 antigen is present on all cells of hematopoietic origin, but not erythrocytes

Background:

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA). CD45 plays a key role in TCR and BCR signal transduction.

Immunogen:

Mouse lymphoma cell line

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Flow Cytometry, IHC (Frozen), IHC (Paraffin)

Application Notes:

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Antibodies against the same target:

Rat anti Mouse/Human CD45R In Vivo Antibody - Low Endotoxin [RA3-6B2] (ICH1130), Rat anti Mouse/Human CD45R In Vivo Antibody - Ultra Low Endotoxin [RA3-6B2] (ICH1130UL)

Alternative Names: