Anti-Mouse CD27 (RM27-3E5) In Vivo Antibody - Low Endotoxin

Mouse

CD27

>1mg/ml

Rat IgG1 Kappa

Rat

Varlilumab Biosimilar - Research Grade (ICH5022), Boserolimab Biosimilar - Research Grade (ICH5411)

Aggregation level ≤ 5%

Mouse CD27-human IgG1 Fc fusion protein

CD27 is expressed on T cells, B cells, and NK cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P41272

Blue ice

This product is a purified rat anti-mouse CD27 monoclonal antibody, clone RM27-3E5, of the rat IgG1 kappa isotype. Raised in rat against mouse CD27, it recognises the murine antigen and is validated for functional assays, flow cytometry and immunoprecipitation. The rat IgG1 backbone and defined clone make it well suited to in-vivo immunology and immuno-oncology studies in mouse models, where CD27 engagement is used to probe T-cell costimulation and antitumour immunity. The preparation is produced at low endotoxin levels (research grade below 1 EU/mg; ultra-low options below 0.5 EU/mg) to minimise confounding innate activation during animal dosing, and is supplied in bulk milligram-to-gram quantities appropriate for repeated-injection and multi-cohort experiments. All material is for research use only and is not intended for diagnostic or therapeutic applications. As with any functional-grade reagent, users should confirm activity, working concentration and lot suitability in their own system before scaling into full studies, and pair in-vivo work with appropriate isotype-matched control antibodies. CD27 (TNFRSF7; UniProt P41272) is a member of the tumour necrosis factor receptor superfamily and functions as a costimulatory receptor for T lymphocytes. It is constitutively expressed on most resting T cells, on subsets of NK cells and memory B cells, and its expression is upregulated following activation. Its single ligand is CD70 (TNFSF7), whose expression is tightly restricted to activated immune cells. Engagement of CD27 by CD70 recruits TRAF2 and TRAF5 adaptors, driving NF-kB and JNK signalling that promotes T-cell survival, clonal expansion, effector differentiation and the generation and maintenance of memory. CD27 costimulation supports both CD4 and CD8 responses and contributes to NK-cell and antibody responses. Because agonistic CD27 signalling can amplify antitumour T-cell immunity, the CD27-CD70 axis is an established target for costimulatory immunotherapy.

CD27L receptor, Tnfrsf7, RM27 3E5, RM273E5

RM27-3E5

RM27-3E5 activity is directed against mouse CD27

This monoclonal antibody was purified using Protein G

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Functional Assays, Flow Cytometry, ImmunoPrecipitation

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone RM27-3E5 suitable for in vivo CD27 studies?

A: Yes - RM27-3E5 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD27 studies and in vitro work.

Q: Can I order anti-mouse CD27 clone RM27-3E5 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone RM27-3E5 target?

A: It is an anti-mouse CD27 monoclonal used to study CD27-CD70 costimulatory signalling on T cells; for research use only.