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Murinized anti-Mouse PD-1 (RMP1-14) Recombinant Antibody LALAPG
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Murinized anti-Mouse PD-1 Recombinant Antibody (RMP1-14) LALAPG
Product Benefits:
ichorbio has murinized the popular anti-mouse PD-1 clone RMP1-14 to reduce the immune response when it is used in mice in vivo. From there, we have added a LALAPG mutation to reduce the immune effector function. ichorbio's murinized anti-PD-1 recombinant antibody (RMP1-14) LALAPG is manufactured in a cGMP compliant facility. Click here for ichorbio's RMP1-14 antibodies and here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars. ichorbio: the best antibodies for in vivo research.
Size:
ICH1182LALAPG mouse anti-mouse RMP1-14 is available in the following sizes: 5mg, 10mg, 20mg and 50mg
Target:
PD-1
Clone:
RMP1-14
Isotype:
Mouse IgG2a
Other Names:
Programmed cell death protein 1, Pdcd1, CD279
Uniprot:
Host:
CHO
Species Reactivity:
Mouse
Specificity:
Murinized anti-mouse PD-1 antibody (RMP1-14) recognizes an epitope on Mouse PD-1. ICH1182LALAPG contains the LALAPG mutation to abolish its effector function.
Purification Method:
This recombinant antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:
Subset of double negative thymocytes, activated T and B cells
Background:
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.
Immunogen:
Mouse PD-1 transfected BHK cells
Concentration:
1.0 - 5.0 mg/ml
Formulation:
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:
>95% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
Storage:
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:
Western Blot, Blocking, Flow Cytometry, Functional Assays
Use:
Products are for research use only.
Original Antibody:
Alternative Names:
- CD279 antibody
- CD279 antigen antibody
- mPD 1 antibody
- mPD l antibody
- mPD-1 antibody
- hSLE1 antibody
- PD 1 antibody
- PD-1 antibody
- PD1 antibody
- PDCD 1 antibody
- PDCD1 antibody
- Programmed cell death 1 antibody
- Programmed cell death 1 protein antibody
- Programmed cell death protein 1 antibody
- Protein PD 1 antibody
- Protein PD-1 antibody
- SLEB2 antibody
- Systemic lupus erythematosus susceptibility 2 antibody
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Mouse IgG2a |
| Host | CHO |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Western Blot, Blocking, Flow Cytometry, Functional Assays |
| Storage | Mouse anti-mouse PD-1 antibody (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Mouse anti-PD-1 antibody (RMP1-14) is a recombinant antibody where the original Rat IgG2a backbone of clone RMP1-14 has been changed to Mouse IgG2a. This antibody recognizes an epitope on Mouse PD-1 |
| Species Reactivity | Mouse |
| Target | PD-1 |
| Shipping Conditions | Blue ice |
| background | CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are memb |
| Other names | Programmed cell death protein 1, Pdcd1, CD279 |
| clone | RMP1-14 |
| UniProt | Q02242 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | Subset of double negative thymocytes, activated T and B cells |
| Immunogen | Mouse PD-1 transfected BHK cells |
| Aggregation | Aggregation level ≤ 5% |
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