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Bulk Human IgG4 (IB4) Isotype Control S228P
$750.00 - $6,503.00
Bulk Human IgG4 (IB4) Isotype Control S228P
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Species Reactivity
Human
Target
IgG4
Concentration
>1mg/ml
Isotype
Human IgG4 S228P Kappa
Host
CHO
Aggregation
Aggregation level ≤ 5%
Immunogen
Hen Egg Lysozyme (HEL)
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Uniprot
P01861
Rrid
RRID:AB_2921642
Shipping Conditions
Blue ice
Background
Clone IB4 bearing the S228P mutation is a human IgG4 isotype control in which the hinge is stabilised against Fab-arm exchange, yielding a robust subclass-matched negative reference. Because IB4 S228P carries no relevant specificity, it provides the background and gating baseline that separates genuine antigen engagement from scaffold-driven signal in flow cytometry and functional assays. The anti-IgG4 control clone IB4 is supplied in a low-endotoxin format suitable for in vivo use and manufactured in scalable milligram to gram quantities to serve bulk and high-throughput laboratories. This is a research-use-only, non-therapeutic reagent.
Other Names
Ig gamma-4 chain C region, Immunoglobulin heavy constant gamma 4, IGHG4
Clone
IB4
Specificity
This Human IgG4 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG4 antibody is suitable for in vivo applications. This Human IgG4 isotype control contains the S228P mutation
Purification Method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Fc Modification
S228P
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications
Flow Cytometry, Functional Assays
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Bio X Cell Code
CP147, CP148, CP152, CP181, CP183
Suggested H1
Human IgG4 (IB4) Isotype Control S228P
Faq (q&a)
Q: Is clone IB4 suitable for in vivo use?
A: Yes - IB4 is supplied in a low-endotoxin, in vivo-grade format for use as a matched Human IgG4 negative control in in vivo and in vitro studies.
Q: Can I order the IB4 isotype control in bulk?
A: Yes, it is manufactured in scalable milligram to gram quantities to support high-throughput and bulk experiments.
Q: What is clone IB4 used for?
A: It is a non-targeting Human IgG4 antibody used as an isotype-matched negative control to assess background and Fc effects; for research use only (RUO).
Write Your Own Review
Is clone IB4 suitable for in vivo use?
Yes - IB4 is supplied in a low-endotoxin, in vivo-grade format for use as a matched Human IgG4 negative control in in vivo and in vitro studies.
Can I order the IB4 isotype control in bulk?
Yes, it is manufactured in scalable milligram to gram quantities to support high-throughput and bulk experiments.
What is clone IB4 used for?
It is a non-targeting Human IgG4 antibody used as an isotype-matched negative control to assess background and Fc effects; for research use only (RUO).
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