Anti-Mouse CD11b (M1/70) In Vivo Antibody

Bulk anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70)

Bio X Cell:

ICH1049 is up to 37% cheaper for industry than the equivalent product BE0007 from Bio X Cell. Product Benefits:

ichorbio's anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD11b

Clone:

M1/70

Size:

ichorbio's M1/70 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CD11b M1/70 clone - please contact us for pricing.

Isotype:

Rat IgG2b

Other Names:

Integrin alpha-M, Itgam, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1

Uniprot:

P05555

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) recognizes the (Mr 170 kD) alpha m subunit of Mac-1 (CD11b/CD18, alpha m beta 2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).

Antigen Distribution:

The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.

Immunogen:

B10 mouse spleen cells enriched for T cells

Concentration:

0.5 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), CyTOF, Immunocytochemistry

Application Notes:

Functional Assay: Activation of mouse peritoneal macrophages Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

Antibodies against the same target:

Anti-CD11b In Vivo Antibody - Low Endotoxin [44] (ICH1007)

Immunofluorescence of frozen tissue sections

Sample: Frozen sections of tumor tissues from tumor bearing C57BL / 6 mice (inoculated with LLC cells) Protocol: 1. Tumors were dissected, fixed in 4% paraformaldehyde, and dehydrated in 30% sucrose; 2. Frozen tumor sections were prepared at 25 ℃ and rinsed in PBS; 3. Blocking buffer: PBS containing 0.3% Triton + 5% goat serum; Sections were blocked for 1h; 4. Primary antibodies: Diluted in blocking buffer; incubated overnight at 4℃. Final concentration of ichorbio CD11b antibody clone M1/70 (low) 2μg/ml, Final concentration (high) 10μg/ml. Positive signals were detected at both high and low concentrations 5. Washed by PBST; Secondary antibodies: incubated at 4℃ for 6h; DAPI: 2h Details of secondary antibody: Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) antibody - Jackson Immunoresearch Labs Cat# 112-605-062 - Conc. 7.5μg/ml. 6. Washed by PBST at least 6 times； 7. Add fluorescence decay resistant medium, seal slice； 8. Detected by the laser scanning confocal microscope. Scale bar in the IF figure is 50 μM. Images produced by Dr. Qin from State Key Laboratory of Genetic Engineering, School of Life Sciences of Fudan University

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