Anti-Mouse CD22 (Cy34.1) In Vivo Antibody - Low Endotoxin

Mouse

CD22

>1mg/ml

Mouse IgG1

Mouse

Anti-Mouse CD22 (MB22-11) In Vivo Antibody - Low Endotoxin (ICH1216), Pinatuzumab Biosimilar - Research Grade (ICH5093), Epratuzumab Biosimilar - Research Grade (ICH5194), Inotuzumab Biosimilar - Research Grade (ICH5247), Bectumomab Biosimilar - Research Grade (ICH5379), Mozistobart Biosimilar - Research Grade (ICH5693), Suciraslimab Biosimilar - Research Grade (ICH5884)

Aggregation level ≤ 5%

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P35329

12483

RRID:AB_2921512

Blue ice

Clone Cy34.1 is a mouse anti-mouse CD22 monoclonal antibody of the IgG1 isotype, raised against mouse CD22 (UniProt P35329). Because it recognises native cell-surface CD22 on B lineage cells, it is widely used for flow cytometric identification of B cells and, in functional studies, as a tool for interrogating the CD22 axis and B-cell targeting. This preparation is produced for in vivo and functional research: it is supplied at low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and can be provided in bulk milligram to gram quantities suitable for repeat-dose mouse studies and larger cohorts. As a mouse IgG1 reagent used in a mouse host, it is appropriate for studies where a syngeneic isotype is preferred and where Fc-mediated effector engagement is comparatively limited relative to IgG2a/IgG2b. The antibody is for research use only (RUO) and is not intended for diagnostic or therapeutic use. All content here is guidance for ichorbio to review before publication. CD22 (Siglec-2) is a B-cell-restricted type I transmembrane sialic-acid-binding immunoglobulin-like lectin. It is expressed on mature B cells and is one of the most B-lineage-specific surface markers, largely absent from plasma cells and non-B lineages, which makes it a useful marker and target. CD22 recognises alpha-2,6-linked sialic acid ligands and functions primarily as an inhibitory co-receptor that modulates B-cell receptor (BCR) signalling. Its cytoplasmic tail carries immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that, upon phosphorylation, recruit the phosphatase SHP-1 to dampen BCR-triggered calcium flux and activation, raising the threshold for B-cell responses and contributing to self-tolerance. CD22 also participates in regulating B-cell survival and homing. Because it is B-cell restricted and rapidly internalises upon antibody binding, CD22 is an established target for B-cell-directed depletion and antibody-drug conjugate strategies.

Lyb-8.2, Cy34 1, Cy34-1, Cy341

Cy34.1

Clone Cy34.1 recognizes an epitope on mouse CD22

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Depletion

The suggested concentration for this CD22 (clone Cy34.1) antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0011

Anti-Mouse CD22/Lyb-8.2 (Cy34.1) In Vivo Antibody - Low Endotoxin

Q: Is clone Cy34.1 suitable for in vivo CD22 studies?

A: Yes - Cy34.1 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD22 studies and in vitro work.

Q: Can I order anti-mouse CD22 clone Cy34.1 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone Cy34.1 target?

A: It is an anti-mouse CD22 monoclonal used to study CD22, an inhibitory receptor on B cells; for research use only.