Bulk Anti-Human CD2 (LO-CD2a) In Vivo Antibody

Human

CD2

>1mg/ml

Rat IgG2b Kappa

Rat

Bulk Anti-Human CD2 (G11) Antibody (ICH1001), Siplizumab Biosimilar - Research Grade (ICH5867)

Aggregation level ≤ 5%

LO-CD2a was developed as an immunosuppressive agent for use in organ allotransplantation. LO-CD2a was produced by immunizing a LOU/C rat with purified human T lymphocytes and fusing the splenocytes with the non-secreting rat fusion cell line IR983F, resul

CD2 is expressed on all mature human T cells, most thymocytes, NK cells and a small proportion of bone marrow cells.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P06729

914

RRID:AB_3075632

Blue ice

This is a bulk, in-vivo-ready preparation of the LO-CD2a clone, a rat IgG2b, kappa monoclonal antibody raised against human CD2. The rat IgG2b isotype and defined specificity make it well suited to functional and mechanistic studies where reproducible, lot-consistent reagent is required at scale. LO-CD2a is a widely referenced anti-human CD2 clone with documented immunomodulatory activity, validated here for ELISA, functional assays, and flow cytometry. Because it targets a human antigen, it is typically deployed in humanized-mouse and xenograft systems, ex-vivo human cell assays, and functional T-cell/NK-cell studies rather than conventional immunocompetent mouse models. The product is supplied at low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) to minimize confounding innate activation in functional and in-vivo work, and in bulk milligram-to-gram quantities to support dose-ranging, repeat-dosing, and larger cohort studies. All material is for research use only (RUO). Researchers most often source this reagent by clone name (LO-CD2a) when reproducing or extending published functional CD2-targeting protocols. CD2 (UniProt P06729; also known as LFA-2 or the SRBC/E-rosette receptor) is a ~50 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily expressed predominantly on T cells and natural killer (NK) cells, with expression rising during T-cell maturation. Its principal ligand in humans is CD58 (LFA-3), and CD2-CD58 engagement contributes to adhesion between T cells or NK cells and antigen-presenting or target cells while delivering costimulatory signals that lower the threshold for TCR-driven activation. The cytoplasmic tail couples to intracellular signaling adaptors that reinforce proximal TCR signaling, proliferation, and cytokine production. Because CD2 is highly restricted to and functionally important in T and NK compartments, and because activated and memory effector cells can express elevated CD2, it is an attractive target for modulating or depleting pathogenic lymphocytes in transplantation, autoimmunity, and immuno-oncology contexts.

BTI-322, CD2, CD2 molecule, T11, SRBC, LFA-2, T-cell surface antigen CD2, LFA-3 receptor, rosette receptor, erythrocyte receptor, lymphocyte-function antigen-2, T-cell surface antigen T11/Leu-5, CD2 antigen (p50), sheep red blood cell receptor, LO CD2a, L

LO-CD2a

Anti-CD2 antibody (clone LO-CD2a) activity is directed against human CD2.

This monoclonal antibody was purified using Protein G

Sterile, preservative-free, solution in PBS. BSA and Azide free.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

ELISA, Functional Assays, Flow Cytometry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0406

Q: Is clone LO-CD2a suitable for in vivo CD2 studies?

A: Yes - LO-CD2a is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD2 studies and in vitro work.

Q: Can I order anti-human CD2 clone LO-CD2a in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone LO-CD2a target?

A: It is an anti-human CD2 monoclonal used to study CD2 on T cells and NK cells; for research use only.