Mouse Anti-Mouse PD-1 Antibody (RMP1-14) D265A

ichorbio’s mouse anti-mouse PD-1 antibody (RMP1-14) D265A is manufactured in a cGMP compliant facility. Click here for ichorbio’s RMP1-14 antibodies and here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars. ichorbio: the best antibodies for in vivo research.

ICH1182D265A mouse anti-mouse RMP1-14 is available in the following sizes:
5mg, 10mg, 20mg and 50mg

PD-1

RMP1-14

Mouse IgG1

Programmed cell death protein 1, Pdcd1, CD279

Q02242

CHO

Mouse

Mouse anti-mouse PD-1 antibody (RMP1-14) recognizes an epitope on Mouse PD-1. ICH1182D265A contains a mutation at D265A to reduce its effector function.

This recombinant antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Subset of double negative thymocytes, activated T and B cells

CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.

Mouse PD-1 transfected BHK cells

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Western Blot, Blocking, Flow Cytometry, Functional Assays

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG1 Isotype Control for In Vivo – Low Endotoxin (HKSP84) [ICH2248]

Anti-PD-1 In Vivo Antibody – Low Endotoxin [RMP1-14]

Anti-PD-1 In Vivo Antibody – Low Endotoxin [29F.1A12] (ICH1091), Anti-PD-1 In Vivo Antibody – Ultra Low Endotoxin [29F.1A12] (ICH1091UL)