Bulk anti-Fas Ligand In Vivo Antibody – Low Endotoxin (MFL3)
ichorbio’s anti-Fas Ligand In Vivo Antibody – Low Endotoxin (MFL3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s MFL3 in vivo antibody is available in the following bulk sizes:1mg, 5mg, 25mg, 50mg and 100mgichorbio regularly manufactures multi-gram amounts of our anti-Fas Ligand MFL3 clone – please contact us for pricing.
Armenian Hamster IgG
FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6, CD178
FasL is expressed on activated T cells, NK cells, the eye, and testis.
Anti-Fas Ligand In Vivo Antibody – Low Endotoxin (MFL3), recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells). Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites. FasL also contributes to CD8 proliferation and neutrophil recruitment. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis.
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
<1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:Mycoplasma sppMycoplasma pulmonisPneumonia virus of miceKilham’s rat virusToolan’s H1 virusHamster parvovirusLymphocytic choriomeningitisMinute virus of miceTheiler’s murine encephalomyelitis virusSendai virusReovirus 3Hantaan virus
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Flow Cytometry, Blocking, Immunofluorescence
The suggested concentration for this anti-Fas Ligand In Vivo Antibody – Low Endotoxin (MFL3) for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.