Anti-Mouse Fas Ligand (MFL3) In Vivo Antibody - Low Endotoxin

Mouse

Fas Ligand

>1mg/ml

Armenian Hamster IgG

Armenian Hamster

Aggregation level ≤ 5%

B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells

FasL is expressed on activated T cells, NK cells, the eye, and testis

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P41047

Blue ice

This product is clone MFL3, an Armenian hamster IgG monoclonal antibody raised against mouse Fas Ligand (FasL/CD178, UniProt P41047). The Armenian hamster host and format make it well suited to mouse in-vivo and functional studies, since it avoids the isotype and species pitfalls that complicate rat or mouse anti-mouse reagents in immunocompetent animals. MFL3 recognises the mouse antigen and is validated for blocking, flow cytometry, and immunofluorescence, so it can be used both to interrogate FasL expression on cells and to functionally neutralise FasL-mediated signalling in vitro and in vivo. It is supplied low endotoxin (research grade less than 1 EU/mg; ultra-low option less than 0.5 EU/mg), in-vivo-ready, and available in bulk milligram-to-gram quantities to support repeat dosing across cohorts. All material is for research use only. As with any functional reagent, expression levels, model background, and dosing schedule should be confirmed empirically before drawing mechanistic conclusions. Fas Ligand (FasL, also called CD178, CD95L or TNFSF6) is a type II transmembrane protein of the TNF superfamily and the physiological ligand for Fas (CD95/APO-1). Engagement of Fas by trimeric FasL recruits FADD and caspase-8 to assemble the death-inducing signalling complex, triggering the extrinsic apoptotic pathway. FasL is expressed by activated T cells and NK cells, where it mediates cytotoxic killing and activation-induced cell death that helps terminate immune responses, and it contributes to immune privilege at sites such as the eye and testis. FasL exists as membrane-bound and metalloprotease-cleaved soluble forms, which can differ in their apoptotic versus non-apoptotic signalling activity. Dysregulated Fas/FasL signalling is implicated in autoimmunity, lymphoproliferation, tissue injury, and tumour immune evasion, making FasL a target of interest across immunology and oncology.

FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6, CD178

MFL3

Clone MFL3 recognizes an epitope on mouse FasL

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Blocking, Flow Cytometry, Immunofluorescence

The suggested concentration for this Fas Ligand antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone MFL3 suitable for in vivo Fas Ligand studies?

A: Yes - MFL3 is a low-endotoxin, in vivo-grade antibody suitable for in vivo Fas Ligand blocking and in vitro work.

Q: Can I order anti-mouse Fas Ligand clone MFL3 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone MFL3 target?

A: It is an anti-mouse Fas Ligand monoclonal used to study and block Fas/FasL-mediated apoptosis; for research use only.