Human CD16a (176F) Protein (In Vivo Grade, Ultra-Low Endotoxin)

Human

Human CD16a (176F) Protein

Human embryonic kidney (HEK) 293 cells.

P08637

Ambient

The 176F (low-affinity) allotype of human CD16a (FcγRIIIa) is a major activating Fc gamma receptor on NK cells and macrophages that drives antibody-dependent cellular cytotoxicity, and allelic variation at this position strongly influences therapeutic-antibody potency. This recombinant CD16a (176F) ectodomain, expressed in HEK 293 cells, is validated for SPR, BLI and ELISA to support Fc binding kinetics, ADCC characterization and Fc-variant ranking. It is a research-use-only protein supplied in an in vivo grade, ultra-low-endotoxin format and produced at scalable milligram (mg) to gram (g) scale, giving high-throughput and bulk-screening laboratories a dependable allotype-defined CD16a reagent.

Human Fc gamma RIIIa, CD16A, FCGR3A, FCGRIIIA, FCR3A, FCRIIIA, IGFR3A, IGFRIIIA

The sequence of the extracellular domain of human CD16a (Gly 17-Gln 208) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.