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Mouse CD16a-2 Protein - Biotin
Mouse CD16a-2 Protein - Biotin
Endotoxin
<1.0 EU per mg as determined by the LAL method.
Purity
>95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Species Reactivity
Mouse
Target
Mouse CD16a-2 Protein
Host
Human embryonic kidney (HEK) 293 cells
Uniprot
A0A0B4J1G0
Shipping Conditions
Ambient
Background
FcγRIV, encoded here as mouse CD16a-2, is an activating myeloid Fc receptor whose IgG-subclass selectivity makes it especially informative in murine antibody-effector studies. This biotinylated recombinant ectodomain, produced in HEK 293 cells, is built for oriented streptavidin capture in SPR, BLI and ELISA assays measuring subclass-dependent Fc binding. It is a non-therapeutic, research-use-only reagent manufactured to in vivo grade with ultra-low endotoxin. With scalable milligram (mg) to gram (g) production, it provides high-throughput and bulk laboratories the quantities required for systematic kinetic analysis of murine FcγRIV engagement.
Other Names
CD16-2, FCGR4, FCGRIV, FCR4, FCRIV, IGFR4, IGFRIV
Specificity
The sequence of the extracellular domain of mouse CD16-2 (Gly 21-Gln 203) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Formulation
Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Storage
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen
Applications
SPR, BLI, ELISA
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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