Anti-Mouse Ly-6C (HK1.4) In Vivo Antibody - Low Endotoxin

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ICH1111

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Anti-Mouse Ly-6C (HK1.4) In Vivo Antibody - Low Endotoxin

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Concentration	0.5 mg/ml
Isotype	Rat IgG2c
Host	Rat
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Immunoprecipitation: 1ï؟½2 _g per 100ï؟½500 _g of total protein (1 ml of cell lysate). Each investigator should determine their own optimal working dilution for specific applications.
Applications	Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen)
Storage	anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at ï؟½20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw C
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and HPLC
Formulation	No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Purification Method	This monoclonal antibody was purified using Protein G
Specificity	Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) recognizes an epitope on Mouse Ly-6C
Species Reactivity	Mouse
Target	Ly-6C
Shipping Conditions	Blue ice
Other names	Lymphocyte antigen 6 complex, locus C
clone	HK1.4
UniProt	P0CW02
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5
Immunogen	L3 cloned CTL cells
Aggregation	Aggregation level ≤ 5%
Antibodies against the same target	Anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin [RB6-8C5] (ICH1131), Anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin [RB6-8C5] (ICH1131UL), Anti-Ly-6C In Vivo Antibody - Low Endotoxin [7B10] (ICH1095), Anti-Ly-6G In Vivo Antibody - Low Endotoxin [1A8] (ICH1089), Anti-Ly-6G In Vivo Antibody - Ultra Low Endotoxin [1A8] (ICH1089UL)