Bulk Anti-Human CD1d (D5) Antibody

Human

CD1d

>1mg/ml

Mouse IgG2b

Mouse

Anti-Mouse CD1d (20H2) Antibody - Low Endotoxin (ICH1240)

Aggregation level ≤ 5%

CD1d a1-a2 domains

CD1d is expressed on monocytes, DCs, macrophages, and epithelial cells

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P15813

Blue ice

This is a bulk-format anti-human CD1d antibody, clone D5, a mouse monoclonal of the IgG2b isotype raised against human CD1d (UniProt P15813). It is validated for immunoprecipitation and Western blot, making it well suited to biochemical characterisation of CD1d expression, glycosylation and complex formation rather than in-vivo work. Supplied at research scale in bulk milligram-to-gram quantities and as a research-use-only (RUO) reagent, it is manufactured with low endotoxin to support demanding cell-based and functional assays where contaminating LPS would confound results. The IgG2b format and defined clone provide lot-to-lot consistency for reproducible pulldown and blotting workflows. Because CD1d is a human target and the validated applications are biochemical, this reagent is oriented toward mechanistic studies of lipid-antigen presentation, NKT-cell biology and CD1d trafficking in human cells and tissues. Investigators pairing it with functional or flow assays should confirm performance in their own system, as validated applications here are IP and WB. CD1d is a non-classical, MHC class I-like antigen-presenting molecule encoded by the CD1D gene and expressed as a heterodimer with beta-2-microglobulin. Unlike classical MHC molecules, CD1d presents lipid and glycolipid antigens rather than peptides, using a deep hydrophobic groove to bind the acyl chains of self and microbial lipids such as alpha-galactosylceramide and endogenous glycosphingolipids. It is expressed on antigen-presenting cells including dendritic cells, macrophages and B cells, as well as on many epithelial cells. CD1d-lipid complexes are recognised by invariant natural killer T (iNKT) cells and other lipid-reactive T cells, triggering rapid cytokine release that bridges innate and adaptive immunity. Through this axis CD1d contributes to antimicrobial defence, tumour immunosurveillance, autoimmunity and tissue inflammation, and CD1d expression on tumour cells is an area of active immuno-oncology interest.

R3G1

D5

Clone D5 recognizes an epitope on human CD1d

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Immunoprecipitation, Western Blot

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone D5 suitable for in vivo CD1d studies?

A: Yes - D5 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD1d studies and in vitro work.

Q: Can I order anti-human CD1d clone D5 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone D5 target?

A: It is an anti-human CD1d monoclonal used to study lipid antigen presentation to NKT cells; for research use only.