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Bulk Anti-Human CD45 (T29/33) Antibody

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SKU

ICH1173

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$390.00

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Bulk anti-human CD45 antibody (T29/33)

Product Benefits:

ichorbio's bulk anti-human CD45 antibody (T29/33) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD45

Other Names:

LCA, Leukocyte Common Antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Clone:

T29/33

Size:

ichorbio's anti-human CD45 antibody (T29/33) is available in the following bulk sizes:5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of clone T29/33 - please contact us for pricing.

Isotype:

Mouse IgG2b

Host:

Mouse

Species Reactivity:

Human

Specificity:

Clone T29/33 recognizes an epitope on human CD45.

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The CD45 antigen is present on all human leukocytes and progenitors in bone marrow. This antibody binds to all human hematopoietic cells, but not to non-hematopoietic cells. Anti-CD45 (T29/33) is not cytotoxic in the presence of complement.

Concentration:

>2.0 mg/ml

Formulation:

This anti-human CD45 antibody (T29/33) is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Flow Cytometry

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

More Information
Concentration	≤ 5.0 mg/ml
Isotype	Mouse IgG2b
Host	Mouse
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	The suggested concentration for this CD45 antibody for staining cells in flow cytometry is ≤ 1.0 _g per 10^6 cells in a volume of 100 _l. Titration of the reagent is recommended for optimal performance for each application.
Applications	Flow Cytometry
Storage	This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin	<1.0 EU/mg as determined by the LAL method
Purity	≥95% monomer by analytical SEC, >95% by SDS Page
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Purification Method	This monoclonal antibody was purified using Protein A
Specificity	Clone T29/33 recognizes an epitope on human CD45.
Species Reactivity	Human
Target	CD45
Shipping Conditions	Blue ice
background	CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and
Other names	LCA, Leukocyte Common Antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
clone	T29/33
UniProt	P08575
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	The CD45 antigen is present on all human leukocytes and progenitors in bone marrow. This antibody binds to all human hematopoietic cells, but not to non-hematopoietic cells. Anti-CD45 (T29/33) is not cytotoxic in the presence of complement.
Aggregation	Aggregation level ≤ 5%