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Bulk Anti-Human CD45 (T29/33) Antibody
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Bulk Anti-Human CD45 (T29/33) Antibody
| Concentration | ≤ 5.0 mg/ml |
|---|---|
| Isotype | Mouse IgG2b |
| Host | Mouse |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | The suggested concentration for this CD45 antibody for staining cells in flow cytometry is ≤ 1.0 _g per 10^6 cells in a volume of 100 _l. Titration of the reagent is recommended for optimal performance for each application. |
| Applications | Flow Cytometry |
| Storage | This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | <1.0 EU/mg as determined by the LAL method |
| Purity | ≥95% monomer by analytical SEC, >95% by SDS Page |
| Formulation | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Clone T29/33 recognizes an epitope on human CD45. |
| Species Reactivity | Human |
| Target | CD45 |
| Shipping Conditions | Blue ice |
| background | CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and |
| Other names | LCA, Leukocyte Common Antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200 |
| clone | T29/33 |
| UniProt | P08575 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | The CD45 antigen is present on all human leukocytes and progenitors in bone marrow. This antibody binds to all human hematopoietic cells, but not to non-hematopoietic cells. Anti-CD45 (T29/33) is not cytotoxic in the presence of complement. |
| Aggregation | Aggregation level ≤ 5% |
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