Bulk Anti-Human CD45RO (UCHL-1) Antibody

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SKU
ICH1175
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$390.00

Bulk Anti-Human CD45RO (UCHL-1) Antibody

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Concentration ≤ 5.0 mg/ml
Isotype Mouse IgG2a
Host Mouse
Use Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes The suggested concentration for this CD45 antibody for staining cells in flow cytometry is ≤ 1.0 _g per 10^6 cells in a volume of 100 _l. Titration of the reagent is recommended for optimal performance for each application.
Applications CODEXï؟½, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Western Blot
Storage This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin <1.0 EU/mg as determined by the LAL method
Purity ≥95% monomer by analytical SEC, >95% by SDS Page
Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Purification Method This monoclonal antibody was purified using Protein A
Specificity Clone UCHL-1 recognizes an epitope on human CD45
Species Reactivity Human
Target CD45RO
Shipping Conditions Blue ice
background CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and
Other names CD45RO molecule is the 180 kDa isoform of CD45 Leucocyte Common Antigen (LCA)
clone UCHL-1
UniProt P08575
Buffer ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas.
Aggregation Aggregation level ≤ 5%
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