Bulk Anti-Human CD45RO (UCHL-1) Antibody

Human

CD45RO

>1mg/ml

Mouse IgG2a

Mouse

Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin (ICH1130), Bulk Anti-Human CD45 (BC8) Antibody (ICH1155), Bulk Anti-Human CD45 (T29/33) Antibody (ICH1173)

Aggregation level ≤ 5%

The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P08575

Blue ice

This is the widely used anti-human CD45RO clone UCHL-1, a mouse IgG2a monoclonal produced in bulk quantities (mg-g scale) for research applications. Raised against human CD45RO, it recognises the low-molecular-weight isoform of the CD45 (leukocyte common antigen) family and is one of the classic markers for distinguishing memory from naive T cells. The antibody is validated across a broad application range, including CODEX multiplexed imaging, flow cytometry, IHC on both frozen and paraffin-embedded tissue, immunoprecipitation, and Western blot, making it suitable for both single-cell phenotyping and tissue-level spatial studies. Supplied as a research-grade, low-endotoxin preparation for reproducible immunophenotyping and functional in-vitro assays, it is well suited to characterising human memory T-cell populations, mapping immune infiltrates in fixed tissue sections, and correlating protein expression across platforms. As a human-reactive reagent available in bulk, it supports large cohort studies, assay development, and multiplexed panels where consistent lot-to-lot performance matters. For research use only (RUO); not for diagnostic or therapeutic use. CD45RO is a low-molecular-weight isoform of CD45 (PTPRC), the leukocyte common antigen, a transmembrane protein tyrosine phosphatase expressed on all nucleated haematopoietic cells. CD45 isoforms arise through alternative splicing of the variable exons A, B, and C in the extracellular region; CD45RO is the shortest isoform, generated when all three variable exons are excluded. Its intracellular phosphatase activity regulates Src-family kinases such as Lck and Fyn, setting thresholds for antigen-receptor signalling in T and B lymphocytes. Functionally, CD45RO marks antigen-experienced (memory and activated) T cells and is reciprocally expressed with the high-molecular-weight isoform CD45RA, which characterises naive T cells. The switch from CD45RA to CD45RO expression upon T-cell activation makes CD45RO a cornerstone marker for identifying memory T-cell subsets in human immunology, and it is also expressed on subsets of monocytes, macrophages, and granulocytes.

CD45RO molecule is the 180 kDa isoform of CD45 Leucocyte Common Antigen (LCA), UCHL 1, UCHL1

UCHL-1

Clone UCHL-1 recognizes an epitope on human CD45

This monoclonal antibody was purified using Protein A

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

CODEX®, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Western Blot

The suggested concentration for this CD45 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.