Bulk Anti-Human CD45RO (UCHL-1) Antibody

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SKU

ICH1175

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$380.00

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Bulk anti-human CD45RO antibody (UCHL-1)

Product Benefits:

ichorbio's bulk anti-human CD45RO antibody (UCHL-1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD45RO

Other Names:

CD45RO molecule is the 180 kDa isoform of CD45 Leucocyte Common Antigen (LCA)

Clone:

UCHL-1

Size:

Anti-human CD45RO antibody (UCHL-1) is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our clone UCHL-1 - please contact us for pricing.

Isotype:

Mouse IgG2a

Host:

Mouse

Species Reactivity:

Human

Specificity:

Anti-human CD45RO antibody (UCHL-1) recognizes Human CD45.

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.

Concentration:

>2.0 mg/ml

Formulation:

Bulk anti-human CD45RO antibody (UCHL-1) is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Western Blot, CODEX®

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.