Anti-Mouse CD49d (PS/2) In Vivo Antibody - Low Endotoxin

Mouse, Human

CD49d

>1mg/ml

Rat IgG2b Kappa

Rat

Natalizumab Biosimilar - Research Grade (ICH4037)

Aggregation level ≤ 5%

PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0.

CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P13612;Q00651

RRID:AB_3075644

Blue ice

This is the PS/2 clone, a rat IgG2b kappa monoclonal raised against mouse CD49d (integrin alpha-4). It is one of the most widely used tools for studying alpha-4 integrin function in the mouse and is offered here in a format built for in-vivo and functional work: low endotoxin (research grade <1 EU/mg, >95% pure; ultra-low option <0.5 EU/mg, 98% pure), supplied in scalable milligram-to-gram quantities for repeat dosing across cohorts. PS/2 is a function-blocking antibody: it binds the alpha-4 subunit and interferes with alpha-4 integrin (VLA-4 and alpha-4/beta-7) engagement of its ligands, making it suited to leukocyte-adhesion and trafficking studies as well as autoimmune and inflammation models. Beyond in-vivo dosing, the clone is validated for flow cytometry, immunofluorescence, and immunohistochemistry, so the same reagent can be used to confirm target engagement and to phenotype tissues. All material is research use only (RUO) and intended for animal and in-vitro research, not therapeutic use. CD49d is integrin alpha-4 (ITGA4). It pairs with beta-1 (CD29) to form VLA-4 (alpha-4/beta-1) and with beta-7 to form the alpha-4/beta-7 integrin. VLA-4 binds VCAM-1 on activated endothelium and the CS-1 region of fibronectin, mediating firm adhesion, rolling, and transendothelial migration of lymphocytes, monocytes, eosinophils, and other leukocytes into inflamed tissue. Alpha-4/beta-7 binds MAdCAM-1 and directs lymphocyte homing to gut-associated lymphoid tissue. Because these interactions control leukocyte recruitment, alpha-4 integrin is central to inflammatory and autoimmune disease; it is the target of the clinical antibody natalizumab in humans. CD49d is expressed on most mononuclear leukocytes and is also used as an activation/memory marker on T cells. The listed UniProt accessions are mouse ITGA4 (P13612) and human ITGA4 (Q00651).

VLA-4α, ITGA4, Integrin α4

PS/2

PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.

This monoclonal antibody was purified using Protein G

Sterile, preservative-free, solution in PBS. BSA and Azide free.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Depletion, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

BE0071

Anti-Mouse CD49d/VLA-4α (PS/2) In Vivo Antibody - Low Endotoxin

Q: Is clone PS/2 suitable for in vivo CD49d studies?

A: Yes - PS/2 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD49d blocking and in vitro work.

Q: Can I order anti-mouse CD49d clone PS/2 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone PS/2 target?

A: It is an anti-CD49d monoclonal used to study and block the alpha-4 integrin chain CD49d; for research use only.