Bulk Anti-Human HLA-DR (L243) Antibody

Yuan et al. WNT4 promotes macrophage polarization via granulosa cell M-CSF and reduces granulosa cell apoptosis in endometriosis, Cytokine, 2023, Volume 172, 156400.

Human

HLA-DR

>1mg/ml

Mouse IgG2a

Mouse

Aggregation level ≤ 5%

Unknown

The HLA-DR antigen is present on B-lymphocytes, activated T-lymphocytes and activated natural killer (NK) lymphocytes. This antigen is also expressed on monocytes, macrophages, human progenitor cells and B-cell lymphomas.

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01903;P01911

Blue ice

This is clone L243, a mouse IgG2a monoclonal that recognizes human HLA-DR, one of the classical MHC class II molecules. L243 is a long-established, widely characterized anti-HLA-DR clone that binds a conformational epitope on the assembled alpha/beta heterodimer, making it well suited to studies of antigen-presenting cell biology, HLA-DR expression, and MHC class II function. The antibody is validated across a broad application range including flow cytometry, Western blot, immunoprecipitation, blocking assays, CyTOF, and frozen-section IHC, supporting both phenotyping and functional/mechanistic work. Supplied research-grade with low endotoxin (<1 EU/mg; ultra-low <0.5 EU/mg options) and available in bulk milligram-to-gram quantities, it is intended for demanding in-vitro and functional research where lot-to-lot consistency and scale matter. Because the target is human, this reagent is oriented toward human cell and tissue systems (PBMCs, dendritic cells, B cells, monocytes, cell lines) rather than mouse in-vivo dosing models. For research use only (RUO); not for diagnostic or therapeutic use. HLA-DR is a classical MHC class II molecule (encoded here by the DRA chain, P01903, and a DRB chain, P01911) expressed as an alpha/beta heterodimer on professional antigen-presenting cells including dendritic cells, B lymphocytes, macrophages/monocytes, and thymic epithelium, and inducibly on activated T cells and other cell types. Its physiological role is to present peptides derived from exogenous and endosomal proteins to CD4+ T helper cells via the T-cell receptor, a central step in initiating and shaping adaptive immunity. Peptide loading occurs in the endolysosomal MIIC compartment, facilitated by the invariant chain (CD74) and the HLA-DM chaperone. HLA-DR expression level is a widely used marker of APC activation and, on monocytes, an indicator of immune status; HLA-DR alleles are highly polymorphic and strongly linked to autoimmune disease susceptibility, transplantation matching, and drug hypersensitivity.

Major Histocompatibility Class II, MHC class II, HLA-DR Monomorphic

L243

Anti-HLA-DR In Vivo Antibody - Low Endotoxin (L243) recognizes/reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross-react with HLA-DP and HLA-DQ

This monoclonal antibody was purified using Protein A

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4.

anti-HLA-DR In Vivo Antibody - Low Endotoxin (L243) is stable for at least four (4) weeks when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw

Western Blot, Blocking, Flow Cytometry, Immunoprecipitation, CyTOF, IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone L243 suitable for in vivo blocking?

A: Yes, L243 is supplied in a low-endotoxin, in vivo-grade format suitable for HLA-DR blocking and functional studies as well as in vitro work.

Q: Can I order clone L243 anti-HLA-DR in bulk?

A: Yes, it is produced in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone L243 recognise?

A: L243 is an anti-human HLA-DR monoclonal antibody used to detect this MHC class II molecule on antigen-presenting cells; for research use only.