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Bulk Anti-Human MHC Class I (W6/32) Antibody

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SKU

ICH1023

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$390.00

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Bulk anti-human MHC Class I antibody (W6/32)

Bio X Cell:

ICH1023 is up to 37% cheaper for industry than the equivalent product BE0079 from Bio X Cell.

Product Benefits:

ichorbio's bulk anti-MHC Class I Antibody (W6/32) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

MHC Class I

Clone:

W6/32

Size:

ichorbio's W6/32 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-MHC Class I W6/32 clone - please contact us for pricing.

Isotype:

Mouse IgG2a

Other Names:

Major Histocompatibility Class I, MHC class I

Host:

Mouse

Species Reactivity:

Human

Specificity:

Bulk anti-human MHC Class I antibody (W6/32) recognizes/reacts with major histocompatibility complex (MHC) class I, HLA-A, B, and C

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

Background:

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and plays a central role in cell-mediated immune responses and tumor surveillance.

Immunogen:

Human tonsil cell membrane

Concentration:

1.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assays, IHC (Frozen)

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)

Antibodies against the same target:

Anti-MHC Class I In Vivo Antibody - Low Endotoxin [A4C8.1-Do9] (ICH1097)

Alternative Names:

DKFZp686P19218 antibody
EA1.2 antibody
EA2.1 antibody
HLA 5.4 antibody
HLA 6.2 antibody
HLA A antibody
HLA B antibody
HLA C antibody
HLA CDA12 antibody
HLA F antibody
HLA G antibody
HLA G histocompatibility antigen class I G antibody
HLAF antibody
Major histocompatibility complex class I A antibody
Major histocompatibility complex class I antibody
Major histocompatibility complex class I E antibody
Major histocompatibility complex class I F antibody
MHC antibody
MHC Class I antigen antibody
MHC G antibody

More Information
Concentration	1.0 mg/ml
Isotype	Mouse IgG2a
Host	Mouse
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Applications	Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assays, IHC (Frozen)
Storage	anti-MHC Class I In Vivo Antibody - Low Endotoxin (W6/32) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and HPLC
Formulation	No carrier proteins or preservatives in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl.
Purification Method	This monoclonal antibody was purified using Protein A
Specificity	Anti-MHC Class I In Vivo Antibody - Low Endotoxin (W6/32) recognizes/reacts with major histocompatibility complex (MHC) class I, HLA-A, B, and C
Species Reactivity	Human
Target	MHC Class I
Shipping Conditions	Blue ice
background	MHC class I antigens associated with _2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and plays a central role in cell-mediated immune responses and tumor surve
Other names	Major Histocompatibility Class I, MHC class I
clone	W6/32
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.
Immunogen	Human tonsil cell membrane
Aggregation	Aggregation level ≤ 5%
Antibodies against the same target	Anti-MHC Class I In Vivo Antibody - Low Endotoxin [A4C8.1-Do9] (ICH1097), Anti-MHC Class I In Vivo Antibody - Low Endotoxin [AF6-88.5] (ICH1098)