Anti-Mouse CD120a In Vivo Antibody – Low Endotoxin (55R-593) [ICH1069]

ichorbio’s anti-CD120a In Vivo Antibody – Low Endotoxin (55R-593) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD120a

55R-593

Armenian Hamster IgG1

Tumor necrosis factor receptor superfamily member 1A, Tnfrsf1a, TNF-R1, TNF-RI, p55, p60

P25118

Armenian Hamster

Mouse

Anti-CD120a In Vivo Antibody – Low Endotoxin (55R-593) recognizes Mouse CD120A

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Variety of cell types at low levels

Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-A and TNF-B (also known as LT-A). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein). Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

E. coli-expressed extracellular domain of the mouse TNFRI protein

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Armenian Hamster IgG Isotype Control for In Vivo – Low Endotoxin [PIP] (ICH2251)

Anti-CD120a In Vivo Antibody – Low Endotoxin [55R-170] (ICH1094)