Anti-Mouse CD120a (55R-593) In Vivo Antibody - Low Endotoxin

Mouse

TNF Receptor I

>1mg/ml

Armenian Hamster IgG1

Armenian Hamster

Anti-Mouse CD120a (55R-170) In Vivo Antibody - Low Endotoxin (ICH1094)

Aggregation level ≤ 5%

E. coli-expressed extracellular domain of the mouse TNFRI protein

Variety of cell types at low levels

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P25118

Blue ice

This is clone 55R-593, an Armenian Hamster IgG1 monoclonal antibody raised against mouse CD120a (TNF Receptor I, TNFR1/p55). Because it is generated in Armenian hamster against a mouse target, it is well suited to in-vivo and functional studies in mouse models, where a non-mouse host isotype helps limit unwanted host anti-antibody responses during repeat dosing. The antibody is supplied research-grade with low endotoxin (<1 EU/mg; ultra-low <0.5 EU/mg options), a format specifically intended for in-vivo administration and functional assays where endotoxin contamination would otherwise confound cytokine and inflammatory readouts. It is available in bulk milligram-to-gram quantities to support multi-cohort animal studies and longitudinal dosing schedules. Validated applications include immunoprecipitation and Western blot, making it useful for confirming target engagement and for biochemical characterization of TNFR1 alongside functional work. The product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. All content here is guidance for ichorbio to review before publishing. CD120a, also known as TNF Receptor 1 (TNFR1, p55/p60, gene Tnfrsf1a; UniProt P25118), is one of the two principal receptors for tumor necrosis factor (TNF), the other being TNFR2/CD120b. TNFR1 is expressed broadly across most nucleated cell types and can be engaged by both soluble and membrane-bound TNF. It contains an intracellular death domain, and ligand engagement drives assembly of signaling complexes that can activate NF-kB and MAPK pathways to promote inflammation and cell survival, or, alternatively, recruit death-domain adaptors to trigger apoptosis or necroptosis. Through these outputs TNFR1 is a central mediator of inflammatory responses, host defense, tissue homeostasis, and, when dysregulated, of chronic inflammatory and autoimmune pathology. Because it drives much of TNF's pro-inflammatory and cytotoxic activity, TNFR1 is a key node studied in models of sepsis, autoimmunity, and inflammatory tissue injury.

Tumor necrosis factor receptor superfamily member 1A, Tnfrsf1a, TNF-R1, TNF-RI, p55, p60, CD120a, 55R 593, 55R593

55R-593

Armenian Hamster anti-Mouse CD120A (Clone 55R-593) recognizes Mouse CD120A

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C.

Immunoprecipitation, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 55R-593 suitable for in vivo use?

A: Yes — 55R-593 is supplied in a low-endotoxin, in vivo-grade format suitable for in vivo TNF Receptor I studies as well as in vitro work.

Q: Can I order 55R-593 in bulk or gram quantities?

A: Yes, this anti-mouse TNF Receptor I antibody is manufactured in scalable milligram to gram quantities to support high-throughput and bulk in vivo experiments.

Q: What does clone 55R-593 target?

A: It is an anti-mouse TNF Receptor I monoclonal antibody that binds TNF Receptor I and is used in functional and blocking studies; for research use only (RUO).