Anti-Mouse CD120a (55R-170) In Vivo Antibody, Low Endotoxin

Bulk anti-CD120a In Vivo Antibody - Low Endotoxin (55R-170)

Product Specific Citations

Chuah et al. Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma. Journal of Hepatology (2022).

Product Benefits:

ichorbio's anti-CD120a In Vivo Antibody - Low Endotoxin (55R-170) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD120a

Clone:

55R-170

Isotype:

Armenian Hamster IgG1

Other Names:

Tumor necrosis factor receptor superfamily member 1A, Tnfrsf1a, TNF-R1, TNF-RI, p55, p60

Uniprot:

P25118

Host:

Armenian Hamster

Species Reactivity:

Mouse

Specificity:

Anti-CD120a In Vivo Antibody - Low Endotoxin (55R-170) recognizes an epitope on Mouse TNF RI

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated. TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses. It regulates immune and inflammatory responses.

Immunogen:

Recombinant Mouse TNF RI Extracellular Domain

Concentration:

1.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, Neutralization

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)

Antibodies against the same target:

Anti-CD120a In Vivo Antibody - Low Endotoxin [55R-593] (ICH1069)

Alternative Names: