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Mouse Anti-Mouse PD-1 (29F.1A12) Recombinant Antibody (LALAPG)
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Mouse anti-mouse PD-1 (29F.1A12) Recombinant Antibody - LALAPG
Product Benefits:
This antibody is a recombinant version of ichorbio's bestselling 29F.1A12 clone. We have switched out the original rat constant region and converted it to a murine constant region, and added a LALAPG mutation to abolish Fc associated effector functions.
ichorbio's anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is manufactured in a cGMP compliant facility. Click here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars. ichorbio: the best antibodies for in vivo research.
Target:PD-1
Clone:29F.1A12
Size:ichorbio's 29F.1A12 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 10mg, 20mg. ichorbio regularly manufactures multi-gram amounts of our mouse anti-mouse PD-1 29F.1A12 LALAPG clone - please contact us for pricing.
Isotype:Mouse IgG2a
Other Names:Programmed cell death protein 1, Pdcd1, CD279
Uniprot: Host:Mouse
Species Reactivity:Mouse, Rat
Specificity:Anti-PD-1 recombinant antibody (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Purification Method:This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
Background:Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
Immunogen:PD-1 cDNA followed by PD-1-Ig fusion protein
Concentration:1.0 - 5.0 mg/ml
Formulation:0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:>95% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Storage:
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:Flow Cytometry, Western Blot, Blocking, IHC (Frozen)
Use:Products are for research use only.
Antibodies against the same target:Anti-PD-1 In Vivo Antibody - Low Endotoxin [RMP1-14] (ICH1132), Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [RMP1-14] (ICH1132UL)
Alternative Names:- CD279 antibody
- CD279 antigen antibody
- mPD 1 antibody
- mPD l antibody
- mPD-1 antibody
- mSLE1 antibody
- PD 1 antibody
- PD-1 antibody
- PD1 antibody
- PDCD 1 antibody
- PDCD1 antibody
- Programmed cell death 1 antibody
- Programmed cell death 1 protein antibody
- Programmed cell death protein 1 antibody
- Protein PD 1 antibody
- Protein PD-1 antibody
- SLEB2 antibody
- Systemic lupus erythematosus susceptibility 2 antibody
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Mouse IgG2a |
| Host | Mouse |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per in |
| Applications | Flow Cytometry, Western Blot, Blocking, IHC (Frozen) |
| Storage | Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| Species Reactivity | Mouse |
| Target | PD-1 |
| Shipping Conditions | Blue ice |
| background | Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clone |
| Other names | Programmed cell death protein 1, Pdcd1, CD279 |
| clone | 29F.1A12 |
| UniProt | Q02242 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells |
| Immunogen | PD-1 cDNA followed by PD-1-Ig fusion protein |
| Aggregation | Aggregation level ≤ 5% |
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